Division of Nephrology, the 900th Hospital of Joint Logistic Support Force, PLA, Fuzhou 350025, China.
J Tradit Chin Med. 2021 Feb;41(1):117-124. doi: 10.19852/j.cnki.jtcm.20201228.002.
To evaluate the efficacy of Huanglian root decoction (, HLD) on kidney injury in rat's model of metabolic syndrome (MetS), and investigate the possible mechanism.
A fructose-induced MetS rat model and human renal tubular epithelial cell-line model were used to compare the efficacy of HLD with that of berberine and tauroursodeoxycholic acid (TUDCA). Blood pressure, biochemical parameters, histopathological changes and the expression levels of oxidative stress markers were evaluated in the animal model at the end of an 8-week treatment regimen. Oxidative stress markers and molecules of the signal pathway of endoplasmic reticulum (ER) stress were evaluated in the human cell-line model.
Levels of fasting insulin, systolic blood pressure and diastolic blood pressure were significantly decreased in rats in the Huanglian group compared to those in the MetS group (P < 0.05). Rats treated with HLD and TUDCA exhibited a significant reduction in blood levels of malondialdehyde compared to those in rats in the MetS group (P < 0.05). Significant increases in glutathione peroxidase in human tubular epithelial cells was found in the Huanglian group compared to that in the MetS group (14.02 vs 18.31, P < 0.05). The mRNA expression of protein kinase RNA-like endoplasmic reticulum kinase and eukaryotic translation initiation factor 2 α decreased significantly in Huanglian groups compared with that in the MetS group.
HLD has therapeutic efficacy on kidney injury in the MetS rat's model, and is non-inferior to berberine and TUDCA.
评价黄连汤（HLD）对代谢综合征（MetS）大鼠模型肾损伤的疗效，并探讨其可能的作用机制。
采用果糖诱导的 MetS 大鼠模型和人肾小管上皮细胞系模型，比较黄连汤与小檗碱和牛磺熊去氧胆酸（TUDCA）的疗效。在 8 周治疗结束时，评估动物模型中的血压、生化参数、组织病理学变化和氧化应激标志物的表达水平。在人细胞系模型中评估氧化应激标志物和内质网（ER）应激信号通路的分子。
与 MetS 组相比，黄连组大鼠的空腹胰岛素、收缩压和舒张压水平显著降低（P<0.05）。与 MetS 组相比，黄连汤和 TUDCA 治疗组大鼠血液中丙二醛水平显著降低（P<0.05）。与 MetS 组相比，黄连组人肾小管上皮细胞中的谷胱甘肽过氧化物酶显著增加（14.02 比 18.31，P<0.05）。与 MetS 组相比，黄连组蛋白激酶 RNA 样内质网激酶和真核翻译起始因子 2α 的 mRNA 表达显著降低。
黄连汤对 MetS 大鼠模型的肾脏损伤具有治疗作用，且与小檗碱和 TUDCA 相当。
