Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Agriculture & Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, South China Agricultural University, Wushan Road 483, Tianhe District, Guangzhou, Guangdong Province 510642, China.
J Agric Food Chem. 2021 Feb 10;69(5):1684-1692. doi: 10.1021/acs.jafc.0c06800. Epub 2021 Jan 31.
In recent years, deoxynivalenol (DON) has frequently been detected in wheat grains and their products. The enantioselective impact of flutriafol on the growth and DON biosynthesis of was investigated in relation to water activity (α, 0.97 and 0.99) and temperature (20, 25, and 30 °C) on the wheat-based medium. -(-)-flutriafol exhibited higher bioactivity than -(+)-flutriafol and -flutriafol under the above conditions. Flutriafol enantiomers reduced or stimulated DON biosynthesis depending on α. DON levels were negligible after 14 or 7 days of incubation times under 0.97 and 0.99 a, respectively. Quantitative real-time polymerase chain reaction (qRT-PCR) analyses showed that the expression levels of trichothecene biosynthetic () genes of under 0.97 a were significantly higher than those under 0.99 a. In addition, -(-)-flutriafol can induce more gene expression than -(+)-flutriafol. Taken together, this study indicated that a and temperature play important roles in regulating DON biosynthesis in with flutriafol enantiomers.
近年来，脱氧雪腐镰刀菌烯醇（DON）频繁出现在小麦籽粒及其制品中。本文研究了水活度（α，0.97 和 0.99）和温度（20、25 和 30°C）对含小麦培养基中，氟环唑对的生长和 DON 生物合成的立体选择性影响。在上述条件下，-(-)-氟环唑比 -(+)-氟环唑和 -氟环唑表现出更高的生物活性。氟环唑对映异构体根据 α 减少或刺激 DON 生物合成。在 0.97 和 0.99 a 下分别孵育 14 或 7 天后，DON 水平可忽略不计。实时定量聚合酶链反应 (qRT-PCR) 分析显示，在 0.97 a 下，的三萜烯生物合成（）基因表达水平明显高于 0.99 a。此外，-(-)-氟环唑可诱导比 -(+)-氟环唑更多的基因表达。综上所述，本研究表明，α 和温度在调节含氟环唑对映异构体的 DON 生物合成中起着重要作用。
