Enantioselective Effect of Flutriafol on Growth, Deoxynivalenol Production, and Gene Transcript Levels in .

In recent years, deoxynivalenol (DON) has frequently been detected in wheat grains and their products. The enantioselective impact of flutriafol on the growth and DON biosynthesis of was investigated in relation to water activity (α, 0.97 and 0.99) and temperature (20, 25, and 30 °C) on the wheat-based medium. -(-)-flutriafol exhibited higher bioactivity than -(+)-flutriafol and -flutriafol under the above conditions. Flutriafol enantiomers reduced or stimulated DON biosynthesis depending on α. DON levels were negligible after 14 or 7 days of incubation times under 0.97 and 0.99 a, respectively. Quantitative real-time polymerase chain reaction (qRT-PCR) analyses showed that the expression levels of trichothecene biosynthetic () genes of under 0.97 a were significantly higher than those under 0.99 a. In addition, -(-)-flutriafol can induce more gene expression than -(+)-flutriafol. Taken together, this study indicated that a and temperature play important roles in regulating DON biosynthesis in with flutriafol enantiomers.