Faculty and Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan.
Division of Materials Science and Engineering, Faculty of Engineering, Hokkaido University, Sapporo, Japan.
Artif Cells Nanomed Biotechnol. 2021 Dec;49(1):20-29. doi: 10.1080/21691401.2020.1865388.
Direct contact between bone and implant materials is required for dental implants. Titanium is used for the implant material owing to its mechanical and biological properties. The anodisation as the surface treatment was employed to enhance osteogenesis around titanium. Moreover, carbon nanohorn (CNH), a type of nanometer-sized carbon material, was reported to promote the bone formation. Thus, it is expected that if the surface of anodised Ti (AnTi) is modified with CNHs, Ti-bone contact would be enhanced. In this study, the Ti surface was modified with CNHs by electrophoresis and obtained anodised titanium coated with CNHs (CNH/AnTi). , CNH/AnTi attracted osteoblastic cells more than AnTi, thereby the proliferation of osteoblastic cell was enhanced by CNH/AnTi more than by AnTi. at 7 and 28 days after implantation of CNH/AnTi or AnTi into the rat femur, more aggressive bone formation was observed on the surface of CNH/AnTi than on AnTi. More importantly, the area where newly formed bone tissue directly attached to CNH/AnTi was significantly larger than that for AnTi, suggesting that "contact osteogenesis" was accelerated on CNH/AnTi during the early post-implantation period. CNH/AnTi would be advantageous especially for the early stages of bone regeneration after surgery.
种植牙需要在骨与种植体材料之间直接接触。由于钛具有机械和生物学特性，因此被用作种植体材料。采用阳极氧化作为表面处理方法来增强钛周围的成骨作用。此外，碳纳米角（CNH）是一种纳米级碳材料，据报道可促进骨形成。因此，如果对阳极氧化钛（AnTi）的表面进行碳纳米角（CNH）修饰，预计钛与骨的接触会得到增强。在这项研究中，通过电泳将 CNH 修饰到 Ti 表面上，得到了涂覆有 CNH 的阳极氧化钛（CNH/AnTi）。研究表明，CNH/AnTi 比 AnTi 更能吸引成骨细胞，从而使成骨细胞的增殖通过 CNH/AnTi 得到增强。在将 CNH/AnTi 或 AnTi 植入大鼠股骨 7 和 28 天后，在 CNH/AnTi 表面观察到比 AnTi 表面更具侵略性的骨形成。更重要的是，新形成的骨组织直接附着在 CNH/AnTi 上的区域明显大于 AnTi，这表明在植入后的早期阶段，“接触成骨”在 CNH/AnTi 上得到了加速。CNH/AnTi 尤其有利于手术后早期的骨再生。
