Tardif R, Talbot D, Gérin M
Département de médecine, Faculté de médecine, Université de Montréal, Québec, Canada.
J Anal Toxicol. 1988 Jan-Feb;12(1):42-4. doi: 10.1093/jat/12.1.42.
A gas chromatographic assay specific for the analysis of bromophenylmercapturic acids in urine is described. The mercapturic acids are first hydrolyzed to form bromothiophenols, which are acetylated, extracted into dichloromethane, and analyzed by gas chromatography with a flame ionization detector. Using samples from rats and mice treated with bromobenzene, the authors make a comparison between this specific method and a nonspecific assay for the determination of total thioethers in urines.
本文描述了一种专门用于分析尿液中溴苯基硫醚氨酸的气相色谱测定法。首先将硫醚氨酸水解形成溴代苯硫酚，然后将其乙酰化，萃取到二氯甲烷中，并用火焰离子化检测器进行气相色谱分析。作者使用用溴苯处理过的大鼠和小鼠的样本，对这种特定方法与测定尿液中总硫醚的非特异性测定法进行了比较。
