Biondi P A, Fedele G, Motta A, Secchi C
Clin Chim Acta. 1979 Jun 1;94(2):155-61. doi: 10.1016/0009-8981(79)90008-1.
3,4-Dihydroxyphenylethyleneglycol, a major metabolite of noradrenaline in rat brain, is estimated alone or with 4-hydroxy-3-methoxyphenylethyleneglycol in rat and human urine by gas chromatography with a flame ionization detector. The samples are hydrolyzed and extracted at pH 2 with ethyl acetate. Then, to analyze only 3,4-dihydroxyphenylethyleneglycol the reaction with n-butaneboronic acid is carried out directly; if 4-hydroxy-3-methoxyphenylethyleneglycol also has to be estimated, preliminary acetylation in alkaline aqueous solution is performed. The advantages of the specificity due to the reagents used is discussed.
3,4-二羟基苯乙二醇是大鼠脑中去甲肾上腺素的主要代谢产物，可通过带有火焰离子化检测器的气相色谱法单独或与4-羟基-3-甲氧基苯乙二醇一起在大鼠和人类尿液中进行测定。样品在pH 2条件下水解，并用乙酸乙酯萃取。然后，若仅分析3,4-二羟基苯乙二醇，则直接与正丁基硼酸进行反应；若还需测定4-羟基-3-甲氧基苯乙二醇，则在碱性水溶液中进行初步乙酰化。讨论了所用试剂带来的特异性优势。
