瞬时受体电位香草酸基因和蛋白在糖尿病大鼠心脏中的表达。
Expression of transient receptor potential vanilloid genes and proteins in diabetic rat heart.
机构信息
Department of Physiology, Medical Science College of China Three Gorges University, Yichang, China.
Renhe Hospital of China Three Gorges University, Yichang, China.
出版信息
Mol Biol Rep. 2021 Feb;48(2):1217-1223. doi: 10.1007/s11033-021-06182-7. Epub 2021 Feb 1.
Cardiac complications are leading causes of death in diabetic patients. Imbalance of Ca homeostasis is a hallmark of cardiac dysfunction in diabetes, while TRPV channels are non-selective for cations and are permeable to Ca. Our aim was to evaluate the expression levels of TRPV1, TRPV2, TRPV3, TRPV4, TRPV5, and TRPV6 genes and proteins in cardiac tissue at 3 days and 4, 8, and 12 weeks after induction of diabetes. Sprague-Dawley rats were assigned to control and DM groups. DM was induced by intraperitoneal injection of streptozotocin (60 mg/kg). The expression levels of TRPV genes were analyzed by the quantitative reverse transcription polymerase chain reaction, and TRPV proteins were determined by western blotting. Compared to controls, the expression levels of TRPV2, TRPV3, and TRPV6 in diabetic myocardium did not change, while TRPV1 decreased at 4, 8, and 12 weeks, TRPV4 was upregulated at 3 days and 4, 8, and 12 weeks, TRPV5 mRNA increased at 8 and 12 weeks, and TRPV5 protein increased at 4, 8, and 12 weeks. Our findings showed that TRPV1, TRPV4, and TRPV5 are associated with the diabetic heart.
心脏并发症是糖尿病患者死亡的主要原因。钙稳态失衡是糖尿病心脏功能障碍的标志，而 TRPV 通道对阳离子无选择性，并且可渗透钙离子。我们的目的是评估糖尿病诱导后 3 天和 4、8 和 12 周时心脏组织中 TRPV1、TRPV2、TRPV3、TRPV4、TRPV5 和 TRPV6 基因和蛋白的表达水平。Sprague-Dawley 大鼠被分为对照组和 DM 组。DM 通过腹腔注射链脲佐菌素（60mg/kg）诱导。通过定量逆转录聚合酶链反应分析 TRPV 基因的表达水平，通过 Western blot 测定 TRPV 蛋白。与对照组相比，糖尿病心肌中 TRPV2、TRPV3 和 TRPV6 的表达水平没有变化，而 TRPV1 在 4、8 和 12 周时减少，TRPV4 在 3 天和 4、8 和 12 周时上调，TRPV5 mRNA 在 8 和 12 周时增加，TRPV5 蛋白在 4、8 和 12 周时增加。我们的研究结果表明，TRPV1、TRPV4 和 TRPV5 与糖尿病心脏有关。
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