Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Finetech in Medicine Research Center, Iran University of Medical Science, Tehran, Iran.
Lasers Med Sci. 2022 Feb;37(1):335-343. doi: 10.1007/s10103-021-03256-7. Epub 2021 Feb 1.
Nanotechnology-based photothermal therapy (NPTT) is a new emerging modality of cancer therapy. To have the right prediction and early detection of response to NPTT, it is necessary to get rapid feedback from a tumor treated by NPTT procedure and stay informed of what happens in the tumor site. We performed this study to find if proton magnetic resonance spectroscopy (1H-MRS) can be well responsive to such an imperative requirement. We considered various treatment groups including gold nanoparticles (AuNPs), laser, and the combination of AuNPs and laser (NPTT group). Therapeutic effects on CT26 colon tumor-bearing BALB/c mice were studied by looking at alterations that happened in 1H-MRS signals and tumor size after conducting treatment procedures. In MRS studies, the alterations of choline and lipid concentrations and their ratio were investigated. Having normalized the metabolite peak to water peak, we found a significant decrease in choline concentration post-NPTT (from (1.25 ± 0.05) × 10 to (0.43 ± 0.04) × 10), while the level of lipid concentration in the tumor was slightly increased (from (2.91 ± 0.23) × 10 to (3.52 ± 0.31) × 10). As a result, the choline/lipid ratio was significantly decreased post-NPTT (from 0.41 ± 0.11 to 0.11 ± 0.02). Such alterations appeared just 1 day after NPTT. Tumor shrinkage in all groups was studied and significant changes were significantly detectable on day 7 post-NPTT procedure. In conclusion, the study of choline/lipid ratio using 1H-MRS may help us estimate what happens in a tumor treated by the NPTT method. Such an in vivo assessment is interestingly feasible as soon as just 1 day post-NPTT. This would undoubtedly help the oncologists make a more precise decision about treatment planning strategies. Monitoring of the choline/lipid ratio by H-MRS can be helpful for prediction and early detection of response to nano-photo-thermal therapy.
基于纳米技术的光热疗法（NPTT）是一种新兴的癌症治疗模式。为了对 NPTT 反应进行正确的预测和早期检测，有必要从接受 NPTT 治疗的肿瘤中获得快速反馈，并了解肿瘤部位发生的情况。我们进行这项研究是为了确定质子磁共振波谱（1H-MRS）是否能够很好地满足这一迫切需求。我们考虑了各种治疗组，包括金纳米粒子（AuNPs）、激光和 AuNPs 与激光的组合（NPTT 组）。通过观察治疗后 CT26 结肠肿瘤荷瘤 BALB/c 小鼠 1H-MRS 信号和肿瘤大小的变化，研究了治疗效果。在 MRS 研究中，研究了胆碱和脂质浓度及其比值的变化。对代谢物峰与水峰进行归一化后，我们发现 NPTT 后胆碱浓度显著下降（从（1.25±0.05）×10 降至（0.43±0.04）×10），而肿瘤内脂质浓度略有升高（从（2.91±0.23）×10 升至（3.52±0.31）×10）。因此，NPTT 后胆碱/脂质比值显著降低（从 0.41±0.11 降至 0.11±0.02）。这些变化仅在 NPTT 后 1 天出现。研究了所有组的肿瘤缩小情况，NPTT 后 7 天可明显检测到显著变化。总之，使用 1H-MRS 研究胆碱/脂质比值可能有助于我们估计接受 NPTT 治疗的肿瘤内发生的情况。这种体内评估在 NPTT 后仅 1 天即可实现，非常有趣。这无疑将帮助肿瘤学家更精确地制定治疗计划策略。通过 H-MRS 监测胆碱/脂质比值有助于预测和早期检测纳米光热治疗的反应。
