Tsubouchi R, Takeuchi F, Shibata Y
Department of Biochemistry, Aichi Medical University, Japan.
Life Sci. 1988;42(16):1565-70. doi: 10.1016/0024-3205(88)90015-x.
The calcium uptake into renal brush border membrane vesicles, which has been purified from normal or vitamin B6 deficient rat renal cortex by calcium precipitation, was investigated. The values of Km and Vmax were determined to be 1.89 mM and 4.26 nmol of Ca2+/mg of protein per 20s in vitamin B6 deficient rats, respectively. This Vmax was lower than that of normal rats. The chemical compositions of renal brush border membranes did not display a difference in normal and vitamin B6 deficient rats. The amount of brush border membranes isolated from 1 gram of renal cortex in vitamin B6 deficient rats was less than in normal rats.
研究了通过钙沉淀从正常或维生素B6缺乏的大鼠肾皮质中纯化得到的肾刷状缘膜囊泡对钙的摄取。在维生素B6缺乏的大鼠中，Km和Vmax值分别测定为1.89 mM和每20秒每毫克蛋白质4.26 nmol的Ca2+。这个Vmax低于正常大鼠的Vmax。正常和维生素B6缺乏的大鼠肾刷状缘膜的化学成分没有差异。维生素B6缺乏的大鼠从1克肾皮质中分离出的刷状缘膜的量比正常大鼠少。
