维生素B6缺乏对大鼠肠道钙和草酸盐吸收的影响：大鼠刷状缘膜的化学成分

Effect of pyridoxine deficiency on intestinal absorption of calcium and oxalate: chemical composition of brush border membranes in rats.

作者信息

Farooqui S, Nath R, Thind S K, Mahmood A

出版信息

Biochem Med. 1984 Aug;32(1):34-42. doi: 10.1016/0006-2944(84)90006-1.

Abstract

[U-14C]oxalic acid and 45Ca uptake was measured in control and vitamin B6-deficient rats. Calcium and oxalate uptake rates were significantly increased from the intestine of vitamin B6-deficient rats as compared to pair-fed controls. Oxalate uptake in pair-fed control rats follows a passive diffusion. In pyridoxine-deficient rats, the oxalate uptake increases nonlinearly as the oxalate concentration in the incubation medium increased, indicating a two-component system--a saturable sodium-independent uptake and a linear nonsaturable passive-diffusion component. The brush border membrane composition reveals that membrane sialic acid, cholesterol, and protein contents were markedly reduced. These aberrations in the chemical composition of brush border membrane may be responsible for the enhanced oxalic acid uptake in vitamin B6-deficient rats.

摘要

在对照大鼠和维生素B6缺乏的大鼠中测量了[U-14C]草酸和45Ca的摄取。与配对喂养的对照大鼠相比，维生素B6缺乏的大鼠肠道对钙和草酸盐的摄取率显著增加。配对喂养的对照大鼠对草酸盐的摄取遵循被动扩散。在缺乏吡哆醇的大鼠中，随着孵育培养基中草酸盐浓度的增加，草酸盐摄取呈非线性增加，表明存在一个双组分系统——一种可饱和的非钠依赖性摄取和一种线性的非饱和被动扩散组分。刷状缘膜成分显示，膜唾液酸、胆固醇和蛋白质含量显著降低。刷状缘膜化学成分的这些异常可能是维生素B6缺乏的大鼠草酸摄取增加的原因。