Departamento de Reprodução Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, SP 05508270, Brazil.
Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP 13635900, Brazil.
Res Vet Sci. 2021 Mar;135:167-174. doi: 10.1016/j.rvsc.2021.01.015. Epub 2021 Jan 23.
Metritis in dairy cows is classified as a puerperal disease that affects all layers of the uterine epithelium and occurs during the first 21 days after parturition. The objectives of this study were to identify potential predictive biomarkers of metritis, to monitor the biochemical profile of pre-metritic dairy cows, and to evaluate the reproductive performance of dairy cows with metritis. Fifty-five cows without metritis and fifteen cows that showed clinical signs of metritis were enrolled in this study. Blood samples were collected at -21, -14 and - 7 days before calving and at parturition and assayed for urea, creatinine, aspartate aminotransferase (AST), gamma-glutamyltransferase (GGT), total cholesterol, fibrinogen, total calcium, β-hydroxybutyrate (BHB) and non-esterified fatty acids (NEFA) concentrations. Serum fibrinogen on 21, 14 and 7 days prepartum and at parturition was the strongest discriminator between metritic and control cows. Moreover, metritic cows had lower (P < 0.05) concentrations of urea, creatinine, cholesterol and calcium and higher (P < 0.05) serum AST, GGT activities, and serum fibrinogen, BHB and NEFA concentrations when compared to control cows. The number of days to first ovulation, insemination, services per pregnancy and days to pregnancy were greater (P < 0.05) for metritic cows than the control group, and the follicle size was smaller (P < 0.05) for metritic cows than the control group. Our results indicate alterations in the biochemical profile of dairy cows with metritis and the negative impact on the reproductive performance of metritic cows raised in tropical conditions. In addition, this study showed that the serum fibrinogen could be used as screening biomarkers to indicate cows that might have metritis during the postpartum period.
奶牛子宫内膜炎被归类为一种产后疾病，影响子宫上皮的所有层，发生在分娩后 21 天内。本研究的目的是鉴定子宫内膜炎的潜在预测生物标志物，监测前子宫内膜炎奶牛的生化特征，并评估患有子宫内膜炎的奶牛的繁殖性能。本研究纳入了 55 头无子宫内膜炎的奶牛和 15 头出现子宫内膜炎临床症状的奶牛。在分娩前 21、14 和 7 天以及分娩时采集血液样本，测定尿素、肌酐、天门冬氨酸氨基转移酶（AST）、γ-谷氨酰转移酶（GGT）、总胆固醇、纤维蛋白原、总钙、β-羟丁酸（BHB）和非酯化脂肪酸（NEFA）浓度。产前 21、14 和 7 天及分娩时血清纤维蛋白原是区分子宫内膜炎和对照组奶牛的最强判别因素。此外，与对照组相比，子宫内膜炎奶牛的尿素、肌酐、胆固醇和钙浓度较低（P<0.05），血清 AST、GGT 活性以及血清纤维蛋白原、BHB 和 NEFA 浓度较高（P<0.05）。与对照组相比，子宫内膜炎奶牛的首次排卵、配种、妊娠服务和妊娠天数较多（P<0.05），卵泡大小较小（P<0.05）。我们的研究结果表明，患有子宫内膜炎的奶牛的生化特征发生了改变，并且对热带条件下患有子宫内膜炎的奶牛的繁殖性能产生了负面影响。此外，本研究表明，血清纤维蛋白原可用作筛选生物标志物，以指示产后可能患有子宫内膜炎的奶牛。
