Zheng Rong-Er, Ji Jinliang, Wu Jiamin, Zhang Ruijuan, Li Yabin, Yu Xiaoping, Xu Yipeng
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University, Hangzhou 310018, China.
Insects. 2021 Jan 28;12(2):114. doi: 10.3390/insects12020114.
proclotting enzymes (PCEs) belong to the clip domain serine protease (clip-SP) family, which is a characteristic protease family in arthropods. was previously reported to regulate egg production and development in female , but its role in male is unclear. In the present study, qPCR analysis showed that was expressed in three different tissues (gut, testis and fat body). RNAi revealed that ds injection made the male vas deferens thinner and reduced the oviposition level of the females that mated with ds-treated males, causing eggs not to hatch. Furthermore, immunofluorescence staining showed that PCE3 was widely expressed in the male internal genitalia. However, after ds injection, expression of PCE3 was diffuse in the male internal genitalia, whose peripheral cells seemed degraded. Overall, these results indicate that is important for reproduction in male .
促凝血酶（PCEs）属于clip结构域丝氨酸蛋白酶（clip-SP）家族，这是节肢动物中一个具有特征性的蛋白酶家族。此前有报道称其可调节雌性[物种名称未给出]的产卵和发育，但其在雄性[物种名称未给出]中的作用尚不清楚。在本研究中，qPCR分析表明[基因名称未给出]在三种不同组织（肠道、睾丸和脂肪体）中表达。RNA干扰显示，注射ds使雄性输精管变细，并降低了与经ds处理的雄性交配的雌性的产卵水平，导致卵无法孵化。此外，免疫荧光染色显示PCE3在雄性内生殖器中广泛表达。然而，注射ds后，PCE3在雄性内生殖器中的表达呈弥漫性，其周围细胞似乎退化。总体而言，这些结果表明[基因名称未给出]对雄性[物种名称未给出]的繁殖很重要。
