Donetsk national medical university, Kramatorsk, Ukraine.
Georgian Med News. 2020 Dec(309):37-42.
Aim - to determine oral fluid chemical composition and mineralization level in patients with a wedge-shaped defect and intact hard tissues, conduct correlation between the indicators of cervical enamel and oral fluid. We determined Сa2+, РО43-, SO42 using spectrophotometric method, Na+, К+, Al3+ by atomic emission spectrometry, Mg2+, Zn2+ by atomic absorption spectrometry, Cl- using chloride-selective electrode of pH-meter in oral fluid of 22 patients divided into two groups: case - with wedge-shaped defects (2.81+/- 0.73); control - with intact teeth. Correlation of oral fluid and cervical enamel chemical composition of 22 extracted teeth (12 - intact teeth, 10 wedge shape) was made. We used JSM-6490 LV focused beam electron microscope (scanning) with system of energy-dispersive X-ray microanalysis INCA Penta FETх3. Significant differences haven't been found in chemical analyte content and molar ratios (p>0.05) in oral fluid. The level of mineralization potential was 1.64+/-0.11 times higher in patients of the control group (p≤0.05). The values of molar coefficients in teeth enamel with a wedge-shaped defect were high: Mg/Р by 1.63 times, Ca/P by 24%, they were low: Mg/Са - by 4.7 times, Р/Са - by 14% (p≤0.05). The difference in correlation between the chemical composition of oral fluid and enamel of patients in the control group was the presence of an average directly proportional bond strength: Na+ with Cl- and Mg2+, inversely proportional: Na+ with Сa2+ and РО43-, р<0.0001. Correlation between molar coefficients of oral fluid and enamel in the control group was distinguished by the presence of high and moderate correlation: directly proportional - between Са2+/Cl- and Na+/К+, Са2+/Mg2+; Mg2+/Са2+ and РО43-/Са2+, inversely proportional - between Са2+/Mg2+ and РО43-/Са2+, р<0.0001. We have revealed correlations of the chemical composition of enamel and oral fluid which can be used to assess the mineral state of tooth enamel and control the effectiveness of prevention of the initial forms of wedge-shaped defects in terms of mixed saliva in dynamics.
目的 - 确定楔形缺损且硬组织完整患者的口腔液化学成分和矿化水平，并对颈釉质与口腔液的指标进行相关性分析。我们使用分光光度法测定了 Ca2+、PO43-、SO42-，原子发射光谱法测定了 Na+、K+、Al3+，原子吸收光谱法测定了 Mg2+、Zn2+，pH 计的氯离子选择电极测定了 Cl-。我们将 22 名患者分为两组：病例组 - 有楔形缺损（2.81+/-0.73）；对照组 - 牙齿完整。对 22 颗提取牙齿（12 颗完整牙齿，10 颗楔形）的口腔液和颈釉质化学成分进行了相关性分析。我们使用 JSM-6490LV 聚焦束电子显微镜（扫描）和 INCA Penta FETх3 能谱微分析系统。在口腔液中，未发现化学分析物含量和摩尔比有显著差异（p>0.05）。对照组患者的矿化潜能水平高 1.64+/-0.11 倍（p≤0.05）。楔形缺损牙釉质的摩尔系数值较高：Mg/Р 高 1.63 倍，Ca/P 高 24%，较低：Mg/Ca 低 4.7 倍，P/Ca 低 14%（p≤0.05）。对照组患者口腔液化学成分与牙釉质之间的相关性差异在于存在平均直接正相关强度：Na+与 Cl-和 Mg2+，负相关：Na+与 Ca2+和 PO43-，p<0.0001。对照组口腔液和牙釉质摩尔系数之间的相关性差异在于存在高和中度相关性：直接正比 - Ca2+/Cl-和 Na+/K+，Ca2+/Mg2+；Mg2+/Ca2+和 PO43-/Ca2+，负相关 - Ca2+/Mg2+和 PO43-/Ca2+，p<0.0001。我们发现了牙釉质化学成分与口腔液化学成分之间的相关性，这些相关性可用于评估牙釉质的矿物质状态，并在混合唾液的动态变化中控制预防楔形缺损初始形式的有效性。
