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通过甲基化敏感限制实现无亚硫酸氢盐的单细胞表观基因组学和基因组学。

Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction.

机构信息

Department of Medicine I, Medical Center - University of Freiburg, Freiburg, Germany.

Faculty of Medicine, University of Freiburg, Freiburg, Germany.

出版信息

Commun Biol. 2021 Feb 1;4(1):153. doi: 10.1038/s42003-021-01661-w.

DOI:10.1038/s42003-021-01661-w
PMID:33526904
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7851132/
Abstract

Single-cell multi-omics are powerful means to study cell-to-cell heterogeneity. Here, we present a single-tube, bisulfite-free method for the simultaneous, genome-wide analysis of DNA methylation and genetic variants in single cells: epigenomics and genomics of single cells analyzed by restriction (epi-gSCAR). By applying this method, we obtained DNA methylation measurements of up to 506,063 CpGs and up to 1,244,188 single-nucleotide variants from single acute myeloid leukemia-derived cells. We demonstrate that epi-gSCAR generates accurate and reproducible measurements of DNA methylation and allows to differentiate between cell lines based on the DNA methylation and genetic profiles.

摘要

单细胞多组学是研究细胞间异质性的有力手段。在这里，我们提出了一种单管、无亚硫酸氢盐的方法，用于在单细胞中同时进行全基因组范围内的 DNA 甲基化和遗传变异分析：单细胞限制分析的表观基因组学和基因组学（epi-gSCAR）。通过应用这种方法，我们从单个急性髓系白血病衍生细胞中获得了多达 506,063 个 CpG 和多达 1,244,188 个单核苷酸变异的 DNA 甲基化测量值。我们证明 epi-gSCAR 可以生成准确和可重复的 DNA 甲基化测量值，并允许根据 DNA 甲基化和遗传特征区分细胞系。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fab1/7851132/be0598cda74d/42003_2021_1661_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fab1/7851132/5cd0c553f65c/42003_2021_1661_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fab1/7851132/494309ff1314/42003_2021_1661_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fab1/7851132/be0598cda74d/42003_2021_1661_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fab1/7851132/5cd0c553f65c/42003_2021_1661_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fab1/7851132/494309ff1314/42003_2021_1661_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fab1/7851132/be0598cda74d/42003_2021_1661_Fig3_HTML.jpg

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