Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg, Virginia, USA.
J Vet Intern Med. 2021 Mar;35(2):993-996. doi: 10.1111/jvim.16040. Epub 2021 Feb 1.
Bacterial urine culture is recommended in dogs suspected of having urinary tract infection (UTI), but there is expense and delay in obtaining such results.
HYPOTHESIS/OBJECTIVE: To determine the diagnostic performance of a rapid immunoassay (RIA) dipstick for detection of bacteriuria using voided urine from dogs with clinical signs of lower UTI.
Twenty-four client-owned dogs.
Voided urine was collected and the RIA performed within 30 minutes. Urine collected by cystocentesis was submitted for aerobic urine culture. McNemar's test and kappa coefficient were calculated to determine agreement between the 2 tests.
Nine of 21 dogs (43%) had UTI verified by aerobic urine culture. There was 1 false-negative and no false-positive RIA results. Sensitivity, specificity, positive predictive value, and negative predictive value of the RIA were 89%, 100%, 100%, and 92%, respectively.
This RIA is promising for correctly identifying whether or not voided urine samples from dogs with lower urinary tract clinical signs have true bacteriuria in a rapid, inexpensive manner. Additional patients should be enrolled in a similar study to determine if diagnostic performance is robust in a large population.
怀疑患有尿路感染（UTI）的犬需要进行细菌尿液培养，但获得这些结果既昂贵又耗时。
假设/目的：评估一种快速免疫分析（RIA）检测犬下尿路感染时用尿液样本检测菌尿的诊断性能。
24 只患犬。
采集犬的尿液，在 30 分钟内完成 RIA 检测。使用膀胱穿刺术采集的尿液进行需氧尿液培养。通过 McNemar 检验和kappa 系数计算来确定两种检测方法的一致性。
21 只犬中，9 只（43%）通过需氧尿液培养证实为 UTI。RIA 检测结果有 1 例假阴性，无假阳性。RIA 的灵敏度、特异性、阳性预测值和阴性预测值分别为 89%、100%、100%和 92%。
本研究的 RIA 可快速、经济地正确识别是否存在下尿路临床症状的犬的尿液样本中存在真正的菌尿，具有很大的应用前景。应招募更多的患者进行类似的研究，以确定在大样本中诊断性能是否稳健。
