Way Leilani Ireland, Sullivan Lauren A, Johnson Valerie, Morley Paul S
Department of Clinical Sciences, College of Veterinary Medicine, Colorado State University, Fort Collins, CO 80523, USA.
J Vet Emerg Crit Care (San Antonio). 2013 Jan-Feb;23(1):23-8. doi: 10.1111/vec.12012. Epub 2013 Jan 14.
To determine the utility of performing urine Gram stain for detection of bacteriuria compared to routine urine sediment examination and bacterial aerobic urine culture.
Prospective, observational study.
University teaching hospital.
Urine samples acquired via cystocentesis through convenience sampling from 103 dogs presenting to a tertiary referral institution.
All samples underwent routine urinalysis, including sediment examination, as well as urine Gram stain and quantitative bacterial aerobic urine culture.
The urine Gram stain demonstrated improved sensitivity (96% versus 76%), specificity (100% versus 77%), positive predictive value (100% versus 83%), and negative predictive value (93% versus 69%) when identifying bacteriuria, compared to routine urine sediment examination.
The urine Gram stain is highly sensitive and specific when detecting the presence of bacteria in canine urine samples. Gram staining should be considered when bacteriuria is highly suspected and requires rapid identification while bacterial culture is pending.
与常规尿沉渣检查及细菌需氧尿培养相比，确定进行尿革兰氏染色检测菌尿的效用。
前瞻性观察性研究。
大学教学医院。
通过膀胱穿刺术从103只就诊于三级转诊机构的犬只中方便抽样获取的尿液样本。
所有样本均进行常规尿液分析，包括沉渣检查，以及尿革兰氏染色和细菌需氧尿定量培养。
与常规尿沉渣检查相比，尿革兰氏染色在识别菌尿时显示出更高的敏感性（96%对76%）、特异性（100%对77%）、阳性预测值（100%对83%）和阴性预测值（93%对69%）。
尿革兰氏染色在检测犬尿液样本中细菌的存在时具有高度敏感性和特异性。当高度怀疑菌尿且在细菌培养结果未出时需要快速鉴定时，应考虑进行革兰氏染色。
