Aerobic bacterial flora of the prepuce, urethra, and vagina of normal adult dogs.

Staphylococcus aureus and Mycoplasma spp were the most frequently isolated aerobic microorganisms in specimens obtained from the prepuce and the urethra of 20 normal, adult male dogs. Staphylococcus aureus and Streptococcus canis were the most frequently isolated bacteria in specimens collected from the vagina (in the area of the urethral orifice) of 20 normal intact bitches and 20 spayed bitches. A significant difference was not found in the observed frequency of occurrence of any of the microorganisms isolated from the intact vs the spayed bitches.