Liu Yilin, Xie Chunyan, Zhai Zhenya, Deng Ze-Yuan, De Jonge Hugo R, Wu Xin, Ruan Zheng
School of Food Science and Technology, State Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China.
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China and Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Rotterdam, Netherlands.
Food Funct. 2021 Mar 1;12(4):1829-1840. doi: 10.1039/d0fo02533j.
Uridine (UR) is a pyrimidine nucleoside that plays an important role in regulating glucose and lipid metabolism. The aim of this study was to investigate the effect of UR on obesity, fat accumulation in liver, and gut microbiota composition in high-fat diet (HFD)-fed mice. ICR mice were, respectively, divided into 3 groups for 8 weeks, that is, control (CON, n = 12), high fat diet (HFD, n = 16), and HFD + UR groups (0.4 mg mL-1 in drinking water, n = 16). UR supplementation significantly reduced the body weight and suppressed the accumulation of subcutaneous, epididymal, and mesenteric WAT in HFD-fed mice (P < 0.05). Meanwhile, UR also decreased the lipid droplet accumulation in the liver and liver organoids (P < 0.05). In addition, UR supplementation increased bacterial diversity and Bacteroidetes abundance, and decreased the Firmicutes-to-Bacteroidetes ratio in HFD-fed mice significantly (P < 0.05). UR promoted the growth of butyrate-producing bacteria of Odoribacter, unidentified-Ruminococcaceae, Intestinimonas, Ruminiclostridium, and unidentified-Lachnospiraceae. A close correlation between several specific bacterial phyla or genera and the levels of WAT weight, hepatic TC, or hepatic TG genera was revealed through Spearman's correlation analysis. These results demonstrated that UR supplementation could be beneficial by attenuating HFD-induced obesity and nonalcoholic fatty liver disease.
尿苷（UR）是一种嘧啶核苷，在调节葡萄糖和脂质代谢中起重要作用。本研究的目的是探讨UR对高脂饮食（HFD）喂养小鼠的肥胖、肝脏脂肪积累和肠道微生物群组成的影响。将ICR小鼠分别分为3组，为期8周，即对照组（CON，n = 12）、高脂饮食组（HFD，n = 16）和HFD + UR组（饮用水中含0.4 mg mL-1，n = 16）。补充UR可显著降低HFD喂养小鼠的体重，并抑制皮下、附睾和肠系膜白色脂肪组织（WAT）的积累（P < 0.05）。同时，UR还可减少肝脏和肝脏类器官中的脂滴积累（P < 0.05）。此外，补充UR可增加HFD喂养小鼠的细菌多样性和拟杆菌丰度，并显著降低厚壁菌门与拟杆菌门的比例（P < 0.05）。UR促进了产丁酸细菌奥氏杆菌属、未鉴定的瘤胃球菌科、肠道单胞菌属、瘤胃梭菌属和未鉴定的毛螺菌科的生长。通过Spearman相关性分析揭示了几种特定细菌门或属与WAT重量、肝脏总胆固醇（TC）或肝脏甘油三酯（TG）水平之间的密切相关性。这些结果表明，补充UR可能通过减轻HFD诱导的肥胖和非酒精性脂肪性肝病而有益。
