Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, 29 Zhongguancun East Road, Beijing 100190, P.R. China.
University of Chinese Academy of Sciences, No. 19(A) Yuquan Road, Beijing 100049, P.R. China.
Nano Lett. 2021 Feb 24;21(4):1722-1728. doi: 10.1021/acs.nanolett.0c04516. Epub 2021 Feb 2.
Gram-negative bacteria, which possess an impermeable outer membrane, are responsible for many untreatable infections. The lack of development of new relevant antibiotics for over 50 years has increased threats. Peptides are regarded as the most promising alternatives to antibiotics. However, since the activities of existing peptides are not yet comparable to those of current antibiotics, there is an urgent need to improve their antibacterial efficiencies. Herein, we conjugate peptides onto one-dimensional rod-like tobacco mosaic virus (TMV). The peptides on the obtained nanoparticles (peptide-TMV) are hundreds of times superior to free peptides in combating Gram-negative bacteria. Through morphology and gene detection of , it was revealed that following peptide-TMV application, the high osmotic pressure related to membrane damage and the generated reactive oxygen species cause 's death. In addition, peptide-TMV causes a downregulation of biofilm-related genes, inhibiting biofilm formation. This work paves the way to combat Gram-negative bacteria-related infection.
革兰氏阴性菌具有不可渗透的外膜，是许多难以治疗的感染的罪魁祸首。50 多年来，新的相关抗生素的发展一直停滞不前，这增加了威胁。肽类被认为是最有前途的抗生素替代品。然而，由于现有肽类的活性尚不能与现有抗生素相媲美，因此迫切需要提高它们的抗菌效率。在此，我们将肽类偶联到一维棒状烟草花叶病毒（TMV）上。在获得的纳米颗粒（肽-TMV）上的肽类在对抗革兰氏阴性菌方面比游离肽类强数百倍。通过形态学和基因检测，发现肽-TMV 应用后，与膜损伤相关的高渗透压和产生的活性氧会导致的死亡。此外，肽-TMV 会下调与生物膜形成相关的基因，从而抑制生物膜的形成。这项工作为对抗革兰氏阴性菌相关感染铺平了道路。
