三价阳离子增强的抗菌蓝光有效治疗皮肤霉菌感染。
Effective Treatment of Cutaneous Mold Infections by Antimicrobial Blue Light That Is Potentiated by Quinine.
机构信息
Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Vaccine and Immunotherapy Center, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
出版信息
J Infect Dis. 2021 Sep 17;224(6):1069-1076. doi: 10.1093/infdis/jiab058.
BACKGROUND
Cutaneous mold infections commonly result from an array of traumatic injuries that involve direct inoculation of contaminated soil into wounds. Here, we explored the use of antimicrobial blue light (aBL; 405 nm wavelength) and the combination of aBL with quinine hydrochloride (aBL + Q-HCL) for the treatment of cutaneous mold infections.
METHODS
Efficacy of aBL and aBL + Q-HCL in killing clinically important pathogenic molds (Aspergillus fumigatus, Aspergillus flavus, and Fusarium oxyprorum) was investigated. Ultraperformance liquid chromatography identified and quantified endogenous porphyrins in the mold conidia. Finally, a mouse model of dermabrasion wound infected with a bioluminescent variant of A. fumigatus was developed to investigate the efficacy of aBL in treating cutaneous mold infections.
RESULTS
We demonstrated that mold conidia are tolerant to aBL, but Q-HCL enhances efficacy. Transmission electron microscopy revealed intracellular damage by aBL. aBL + Q-HCL resulted in intracellular and cell wall damage. Porphyrins were observed in all mold strains, with A. fumigatus having the highest concentration. aBL and aBL + Q-HCL effectively reduced the burden of A. fumigatus within an established dermabrasion infection and limited recurrence posttreatment.
CONCLUSIONS
aBL and aBL + Q-HCL may offer a novel approach for the treatment of mold infections.
背景
皮肤霉菌感染通常是由于一系列创伤性损伤引起的，这些损伤涉及将受污染的土壤直接接种到伤口中。在这里，我们探讨了使用抗菌蓝光（aBL；405nm 波长）和 aBL 与盐酸奎宁（aBL+Q-HCL）联合治疗皮肤霉菌感染的方法。
方法
研究了 aBL 和 aBL+Q-HCL 对临床上重要的致病霉菌（烟曲霉、黄曲霉和尖孢镰刀菌）的杀灭效果。超高效液相色谱法鉴定和定量了霉菌孢子中的内源性卟啉。最后，建立了一种经皮划痕伤感染生物发光变异烟曲霉的小鼠模型，以研究 aBL 治疗皮肤霉菌感染的疗效。
结果
我们证明霉菌孢子对 aBL 具有耐受性，但 Q-HCL 可提高其疗效。透射电子显微镜显示 aBL 可导致细胞内损伤。aBL+Q-HCL 可导致细胞内和细胞壁损伤。所有霉菌株中均观察到卟啉，其中烟曲霉的浓度最高。aBL 和 aBL+Q-HCL 可有效降低已建立的经皮划痕伤感染中烟曲霉的负担，并限制治疗后复发。
结论
aBL 和 aBL+Q-HCL 可能为治疗霉菌感染提供一种新方法。
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