Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Vaccine and Immunotherapy Center, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
J Infect Dis. 2020 Feb 3;221(4):618-626. doi: 10.1093/infdis/jiz487.
Antimicrobial resistance is a significant concern to public health, and there is a pressing need to develop novel antimicrobial therapeutic modalities.
In this study, we investigated the capacity for quinine hydrochloride (Q-HCL) to enhance the antimicrobial effects of antimicrobial blue light ([aBL] 405 nm wavelength) against multidrug-resistant (MDR) Gram-negative bacteria in vitro and in vivo.
Our findings demonstrated the significant improvement in the inactivation of MDR Pseudomonas aeruginosa and Acinetobacter baumannii (planktonic cells and biofilms) when aBL was illuminated during Q-HCL exposure. Furthermore, the addition of Q-HCL significantly potentiated the antimicrobial effects of aBL in a mouse skin abrasion infection model. In addition, combined exposure of aBL and Q-HCL did not result in any significant apoptosis when exposed to uninfected mouse skin.
In conclusion, aBL in combination with Q-HCL may offer a novel approach for the treatment of infections caused by MDR bacteria.
抗菌药物耐药性对公共卫生构成重大威胁，迫切需要开发新的抗菌治疗方法。
本研究旨在探讨盐酸奎宁（Q-HCL）是否能增强抗菌蓝光（[aBL]405nm 波长）对体外和体内多药耐药（MDR）革兰氏阴性菌的抗菌作用。
我们的研究结果表明，当 Q-HCL 暴露时用 aBL 照射，MDR 铜绿假单胞菌和鲍曼不动杆菌（浮游细胞和生物膜）的失活显著改善。此外，在小鼠皮肤擦伤感染模型中，Q-HCL 显著增强了 aBL 的抗菌作用。此外，当暴露于未感染的小鼠皮肤时，aBL 和 Q-HCL 的联合暴露不会导致任何明显的细胞凋亡。
综上所述，aBL 联合 Q-HCL 可能为治疗 MDR 细菌引起的感染提供一种新的方法。
