Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Departamento de Química, Universidade de São Paulo, São Paulo, Brazil.
J Sep Sci. 2021 Apr;44(8):1662-1693. doi: 10.1002/jssc.202001070. Epub 2021 Feb 17.
In-tube solid-phase microextraction with a capillary column as extraction device can be directly coupled with high-performance liquid chromatography systems (HPLC). The in-tube solid-phase microextraction technique has been continuously developed since it was introduced in 1997. New couplings have also been evaluated on the basis of state-of-the-art HPLC instruments. Different types of capillaries (wall-coated open tubular, porous layer open tubular, sorbent-packed, porous monolithic rods, or fiber-packed) with selective stationary phases (monoliths, magnetic nanoparticles, conducting polymers, restricted access materials, ionic liquids, carbon, deep eutectic solvents, and hybrid materials) have been developed to boost in-tube solid-phase microextraction performance (sorption capacity and selectivity). This technique has been successfully applied to analyze biological samples (serum, plasma, whole blood, hair, urine, milk, skin, and saliva) for therapeutic drug monitoring, to study biomarkers, to detect illicit drugs, to conduct metabolomics studies, and to assess exposure to drugs. This review describes current advances in in-tube solid-phase microextraction extraction devices and their application in bioanalysis.
管内固相微萃取与毛细管柱作为萃取装置，可直接与高效液相色谱系统（HPLC）联用。自 1997 年引入以来，管内固相微萃取技术不断发展。还根据最先进的 HPLC 仪器对新的耦合进行了评估。具有选择性固定相的不同类型的毛细管（壁涂开管、多孔层开管、填料、多孔整体棒或纤维填充）（整体柱、磁性纳米粒子、导电聚合物、限制进入材料、离子液体、碳、深共晶溶剂和杂化材料）已被开发出来，以提高管内固相微萃取的性能（吸附容量和选择性）。该技术已成功应用于分析生物样品（血清、血浆、全血、头发、尿液、牛奶、皮肤和唾液）中的治疗药物监测、研究生物标志物、检测非法药物、进行代谢组学研究以及评估药物暴露。本综述描述了管内固相微萃取萃取装置的最新进展及其在生物分析中的应用。
