Souza Israel D, Oliveira Igor G C, Queiroz Maria Eugênia C
Departamento de Química, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
Departamento de Química, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
Anal Chim Acta. 2021 Jun 22;1165:238110. doi: 10.1016/j.aca.2020.11.042. Epub 2020 Dec 1.
Fiber-in-tube solid-phase microextraction (fiber-in-tube SPME) with short capillary longitudinally packed with fine fibers as extraction device allows direct coupling to high performance liquid chromatography (HPLC) systems to determine weakly volatile or thermally labile compounds. This technique associates the advantages of miniaturized and analytical on-line systems. Major achievements include the use of different capillaries (fused-silica, copper, stainless steel, polyetheretherketone (PEEK), or poly(tetrafluoroethylene) (PTFE)) that are packed with neat fibers (Zylon®, silk, or Kevlar 29®) or fibers (stainless steel, basalt, or carbon) functionalized with selective coatings (aerogels, ionic liquids (ILs), polymeric ionic liquids (PILs), molecularly imprinted polymers (MIPs), layered double hydroxides (LDHs), or conducting polymer). This review outlines the fundamental theory and the innovative extraction materials for fiber-in-tube SPME-HPLC systems and highlights their main applications in environmental and bioanalyses.
管内纤维固相微萃取（fiber-in-tube SPME）采用纵向填充细纤维的短毛细管作为萃取装置，可直接与高效液相色谱（HPLC）系统联用，用于测定弱挥发性或热不稳定化合物。该技术兼具小型化和在线分析系统的优点。主要成果包括使用不同的毛细管（熔融石英、铜、不锈钢、聚醚醚酮（PEEK）或聚四氟乙烯（PTFE）），这些毛细管填充有纯纤维（Zylon®、丝绸或凯夫拉尔29®）或用选择性涂层（气凝胶、离子液体（ILs）、聚合离子液体（PILs）、分子印迹聚合物（MIPs）、层状双氢氧化物（LDHs）或导电聚合物）功能化的纤维（不锈钢、玄武岩或碳）。本文综述了管内纤维SPME-HPLC系统的基本理论和创新萃取材料，并重点介绍了它们在环境和生物分析中的主要应用。
