Department of Biochemistry and Molecular Biology. Faculty of Kinesiology, Cumming School of Medicine, University of Calgary, Calgary, Alberta T2N 1N4, Canada.
Alberta Children's Hospital Research Institute, University of Calgary, Calgary, Alberta T2N 1N4, Canada.
J Proteome Res. 2021 Mar 5;20(3):1630-1638. doi: 10.1021/acs.jproteome.0c00854. Epub 2021 Feb 2.
Pre-pregnancy obesity and excessive gestational weight gain (GWG) are risk factors for future maternal and childhood obesity. Maternal obesity is potentially communicated to the fetus in part by the metabolome, altering the child's metabolic program in early development. Fasting maternal blood samples from 37 singleton pregnancies at 25-28 weeks of gestation were obtained from mothers with pre-pregnancy body mass indexes (BMIs) between 18 and 40 kg/m. Various health measures including GWG, diet, and physical activity were also assessed. At term (37-42 weeks), a venous umbilical cord sample was obtained. Serum metabolomic profiles were measured using nuclear magnetic resonance spectroscopy as well as a gut and metabolic hormone panel. Maternal and cord serum metabolites were tested for associations with pre-pregnancy BMI, GWG, health outcomes, and gut and metabolic hormones. While cord blood metabolites showed no significant correlation to maternal obesity status or other measured health outcomes, maternal serum metabolites showed distinct profiles for lean, overweight, and obese women. Additionally, four serum metabolites, namely, glutamate, lysine, pyruvate, and valine, allowed prediction of excessive GWG when pre-pregnancy BMI was controlled. Metabolic biomarkers predictive of GWG are reported and, if validated, could aid in the guidance of prenatal weight management plans as the majority of pregnancy weight gain occurs in the third trimester.
孕前肥胖和孕期体重过度增加（GWG）是未来母婴肥胖的危险因素。母体肥胖可能通过代谢组部分传递给胎儿，改变儿童在早期发育中的代谢程序。在妊娠 25-28 周时，从体重指数（BMI）在 18 至 40kg/m 之间的 37 例单胎妊娠的母亲中获得了空腹母亲血液样本。还评估了各种健康措施，包括 GWG、饮食和体育活动。在足月时（37-42 周），获得静脉脐血样本。使用核磁共振波谱法以及肠道和代谢激素面板测量血清代谢组学图谱。检测了母体和脐带血清代谢物与孕前 BMI、GWG、健康结果以及肠道和代谢激素的相关性。虽然脐带血代谢物与母体肥胖状况或其他测量的健康结果没有显著相关性，但母体血清代谢物显示出瘦、超重和肥胖女性的不同特征。此外，当控制孕前 BMI 时，四种血清代谢物（谷氨酸、赖氨酸、丙酮酸和缬氨酸）可以预测 GWG 过多。报告了预测 GWG 的代谢生物标志物，如果得到验证，可能有助于指导产前体重管理计划，因为大多数妊娠体重增加发生在孕晚期。
