Scherer Brian, Surrette Christine, Li Hui, Torab Peter, Kvam Erik, Galligan Craig, Go Steven, Grossmann Greg, Hammond Tyler, Johnson Tammy, St-Pierre Richard, Nelson John R, Potyrailo Radislav A, Khire Tejas, Hsieh Kuangwen, Wang Tza-Huei, Wong Pak Kin, Puleo Chris M
General Electric Research, Niskayuna, NY, USA.
Lab Chip. 2021 Mar 21;21(6):1073-1083. doi: 10.1039/d0lc00937g. Epub 2021 Feb 2.
Single-molecule and single-cell analysis techniques have opened new opportunities for characterizing and analyzing heterogeneity within biological samples. These detection methods are often referred to as digital assays because the biological sample is partitioned into many small compartments and each compartment contains a discrete number of targets (e.g. cells). Using digital assays, researchers can precisely detect and quantify individual targets, and this capability has made digital techniques the basis for many modern bioanalytical tools (including digital PCR, single cell RNA sequencing, and digital ELISA). However, digital assays are dominated by optical analysis systems that typically utilize microscopy to analyze partitioned samples. The utility of digital assays may be dramatically enhanced by implementing cost-efficient and portable electrical detection capabilities. Herein, we describe a digital electrical impedance sensing platform that enables direct multiplexed measurement of single cell bacterial cells. We outline our solutions to the challenge of multiplexing impedance sensing across many culture compartments and demonstrate the potential for rapidly differentiating antimicrobial resistant versus susceptible strains of bacteria.
单分子和单细胞分析技术为表征和分析生物样品中的异质性提供了新机遇。这些检测方法通常被称为数字分析，因为生物样品被划分为许多小隔室，且每个隔室包含离散数量的靶标（如细胞）。使用数字分析，研究人员能够精确检测和量化单个靶标，这种能力使数字技术成为许多现代生物分析工具（包括数字PCR、单细胞RNA测序和数字ELISA）的基础。然而，数字分析主要由光学分析系统主导，这些系统通常利用显微镜来分析分区样品。通过实现经济高效且便携的电学检测能力，数字分析的效用可能会得到显著提升。在此，我们描述了一种数字电阻抗传感平台，该平台能够对单个细菌细胞进行直接多重测量。我们概述了应对跨多个培养隔室进行多重阻抗传感挑战的解决方案，并展示了快速区分抗菌耐药菌和敏感菌菌株的潜力。
