Institute of Microbiology, Friedrich Schiller University, Jena, Germany; Microverse Cluster, Friedrich Schiller University, Jena, Germany.
Institute of Microbiology, Friedrich Schiller University, Jena, Germany.
Curr Opin Microbiol. 2021 Apr;60:1-7. doi: 10.1016/j.mib.2021.01.002. Epub 2021 Jan 30.
In the past decades, Caulobacter crescentus has been extensively studied, mostly regarding its dimorphic, asymmetric life cycle. Its distinct mode of reproduction and the need to optimally adapt to ever-changing environmental conditions require tight coordination of gene regulation. Post-transcriptional regulation through non-coding RNAs and RNA-binding proteins constitutes an important layer of expression control in bacteria, but its principles and mechanisms in Caulobacter have only recently been explored. RNA-binding proteins including the RNA chaperone Hfq and ribonuclease RNase E contribute to the activity of regulatory RNAs. Riboswitches and RNA thermometers govern expression of downstream open reading frames, while the small regulatory RNAs CrfA, ChvR and GsrN instead control targets encoded in trans by direct base-pairing interactions.
在过去的几十年里，弯颈菌属（Caulobacter crescentus）得到了广泛的研究，主要集中在其二态性和不对称的生命周期上。它独特的繁殖方式和适应不断变化的环境条件的需要，要求基因调控的紧密协调。通过非编码 RNA 和 RNA 结合蛋白的转录后调控构成了细菌中表达控制的一个重要层次，但弯颈菌属中这一原理和机制直到最近才被探索。包括 RNA 分子伴侣 Hfq 和核糖核酸酶 RNase E 在内的 RNA 结合蛋白有助于调节 RNA 的活性。核酶开关和 RNA 温度计控制下游开放阅读框的表达，而小分子调节 RNA CrfA、ChvR 和 GsrN 则通过直接碱基配对相互作用来控制编码在转录中的靶标。
