School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, Australia; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk 71491, Saudi Arabia.
Department of Endocrinology, Mater Group, South Brisbane, QLD 4101, Australia; Mater Research, The University of Queensland, South Brisbane, QLD 4101, Australia.
Pregnancy Hypertens. 2021 Mar;23:211-219. doi: 10.1016/j.preghy.2021.01.002. Epub 2021 Jan 26.
Preeclampsia is a pregnancy-specific disorder characterized by hypertension and dysfunction of several organs, that is associated with maternal and fetal complications. The human gut microbiota is related to health and disease including hypertension. Alterations in gut microbiota composition can change the short-chain fatty acid profile released by the bacteria and contribute to hypertension and metabolic syndrome. It is unclear if the composition of the gut microbiota is altered in women who develop late-onset preeclampsia. In this study, we investigated the composition of the gut microbiota at 28 weeks gestation in women who developed late-onset (>34 weeks gestation) preeclampsia (DPE) by 16S rRNA gene amplicon sequencing of fecal samples obtained from 213 pregnant women in the SPRING cohort (Study of Probiotics IN Gestational diabetes). Quantitative real-time PCR was used to assess the density of butyrate-producing genes. Gut microbiota composition was compared between women with and without DPE. The abundance of the butyrate-producing Coprococcus genus significantly decreased in DPE. Abundance of Coprococcus is significantly and positively correlated with the abundance of genes encoding the terminal step in bacterial butyrate formation (but and buk). Women with DPE also had significantly reduced levels of serum butyrate prior to the development of symptoms than controls. This study suggests that a reduction in the abundance of butyrate-producing bacteria, and Coprococcus spp. in particular, may contribute to an increased risk of developing preeclampsia in pregnant women.
子痫前期是一种妊娠特有的疾病，其特征为高血压和多个器官功能障碍，与母婴并发症相关。人类肠道微生物群与健康和疾病有关，包括高血压。肠道微生物群组成的改变可以改变细菌释放的短链脂肪酸谱，并导致高血压和代谢综合征。目前尚不清楚在发生晚期子痫前期的女性中，肠道微生物群的组成是否发生改变。在这项研究中，我们通过对 SPRING 队列（妊娠糖尿病中益生菌的研究）中 213 名孕妇的粪便样本进行 16S rRNA 基因扩增子测序，研究了 28 周妊娠时发生晚期（>34 周妊娠）子痫前期（DPE）的女性肠道微生物群的组成。使用定量实时 PCR 来评估产生丁酸盐的基因的密度。比较了 DPE 妇女与无 DPE 妇女之间的肠道微生物群组成。丁酸产生菌 Coprococcus 属的丰度在 DPE 中显著降低。Coprococcus 的丰度与编码细菌丁酸形成终末步骤的基因（but 和 buk）的丰度呈显著正相关。与对照组相比，发生 DPE 的女性在出现症状前的血清丁酸水平也显著降低。本研究表明，丁酸产生菌，特别是 Coprococcus 属的丰度降低，可能导致孕妇子痫前期风险增加。
