Kurland G, Cheung A T, Miller M E, Ayin S A, Cho M M, Ford E W
Department of Pediatrics, University of California Davis, School of Medicine 95616.
Pediatr Res. 1988 Mar;23(3):293-7. doi: 10.1203/00006450-198803000-00013.
Using the technique of bronchoalveolar lavage, we isolated alveolar macrophages (AM) from the lower respiratory tract of newborn (1-4 days of age), infant (6-10 days of age), juvenile (3-6 months of age), and adult rhesus monkeys. The AM thus obtained were assayed in vitro to determine their chemotactic, phagocytic, and candidicidal capabilities. The predominant (greater than or equal to 89%) cell type in bronchoalveolar lavage effluent in all ages was the AM. Chemotaxis, phagocytosis, and killing of Candida albicans were markedly impaired in neonatal AM as compared with those from infants, juveniles, and adults. AM chemotactic activity achieved normal adult values by 6 days of age. Phagocytosis, and to a lesser extent candidicidal activity, were significantly improved in 6-day-old animals, but adult levels were still not achieved even by 6 months of age.
我们采用支气管肺泡灌洗技术，从新生（1至4日龄）、婴儿（6至10日龄）、幼年（3至6月龄）和成年恒河猴的下呼吸道分离出肺泡巨噬细胞（AM）。对由此获得的肺泡巨噬细胞进行体外检测，以确定其趋化、吞噬和杀念珠菌能力。在所有年龄段的支气管肺泡灌洗流出液中，主要（大于或等于89%）细胞类型为肺泡巨噬细胞。与婴儿、幼年和成年动物的肺泡巨噬细胞相比，新生动物的肺泡巨噬细胞的趋化性、吞噬作用和对白色念珠菌的杀伤能力明显受损。肺泡巨噬细胞的趋化活性在6日龄时达到成年正常水平。在6日龄动物中，吞噬作用以及在较小程度上的杀念珠菌活性显著改善，但即使到6月龄仍未达到成年水平。
