Kozłowska-Geller Monika A, Głuszek Stanisław Z, Lewitowicz Piotr
Collegium Medicum of Jan Kochanowski University in Kielce, Poland.
Contemp Oncol (Pozn). 2020;24(4):247-251. doi: 10.5114/wo.2020.102632. Epub 2021 Jan 4.
The p21 participates in the regulation of DNA repair and replication, and modulation of apoptosis as well. After DNA damage, the p53-dependent induction of p21 results in cell cycle arrest or could trigger cell apoptosis. The objective of the study was the assessment of p21 immunoreactivity in rectal cancer and the estimation of relationships with clinical outcome especially as predictor of poor outcome. While applying the ruling in and out criteria, 102 patients were incorporated to the study, with stage I-IV rectal cancer who had undergone surgery in a planned mode during 2005-2011. The follow-up covered 5 years period from surgery date. Conventional immunohistochemistry were performed using antibody against p21 (p21WAF1 (Clone H252) to detect overexpression targeted receptor. The analysis showed no statistically significant differences in the survival curves of patients in groups with immunoreactivity of p21 protein at 0; 1; 2; 3 ( = 0.6453 in the log-rank test), also is not a significant risk factor for death (HR = 0.915, = 0.7842) and for tumor dissemination (HR = 0.94, = 0.9426). Our study leads to the conclusion that the probability of survival does not depend on p21 expression and do not authorize the importance of p21 immunoreactivity in the detection and monitoring of rectal cancer treatment.
p21参与DNA修复和复制的调控，也参与细胞凋亡的调节。DNA损伤后，p53依赖性诱导p21可导致细胞周期停滞或触发细胞凋亡。本研究的目的是评估p21在直肠癌中的免疫反应性，并评估其与临床结局的关系，尤其是作为不良结局的预测指标。在应用纳入和排除标准时，102例I-IV期直肠癌患者被纳入研究，这些患者在2005年至2011年期间接受了计划性手术。随访从手术日期开始，为期5年。使用抗p21抗体（p21WAF1（克隆H252））进行常规免疫组织化学检测以检测靶向受体的过表达。分析显示，p21蛋白免疫反应性为0、1、2、3的患者组生存曲线无统计学显著差异（对数秩检验中P = 0.6453），p21也不是死亡（HR = 0.915，P = 0.7842）和肿瘤播散（HR = 0.94，P = 0.9426）的显著危险因素。我们的研究得出结论，生存率不取决于p21表达，且p21免疫反应性在直肠癌治疗的检测和监测中并不重要。
