Song Bao, Wang Wei, Zheng Yan, Yang Jianshu, Xu Zhongfa
Department of Biochemistry and Molecular Biology, Shandong University School of Medicine, Jinan, China; Department of Basic Laboratory, Shandong Cancer Hospital and Institute, Jinan, China.
Department of Basic Laboratory, Shandong Cancer Hospital and Institute, Jinan, China; School of Medicine and Life Sciences, University of Jinan, Shandong Academy of Medical Sciences, Jinan, China.
J Surg Res. 2015 Jun 1;196(1):130-5. doi: 10.1016/j.jss.2015.02.035. Epub 2015 Feb 19.
P21-activated kinases (PAKs) are small guanosine triphosphate effectors that play critical roles in many fundamental cellular functions, including cytoskeletal reorganization and cell motility. PAKs are widely expressed in a variety of tissues and are often overexpressed in multiple cancer types. The aim of this study was to investigate the relationship between PAK1 and PAK4 and clinicopathologic features of colorectal cancer.
PAK1 and PAK4 expression in colorectal cancer patients were investigated via TaqMan real-time polymerase chain reaction and immunohistochemistry and clinical analysis.
The relative expression levels of PAK1 and PAK4 gene in colorectal carcinoma tissues were significantly higher than those in normal tissues (P < 0.01). PAK4 expression was higher than PAK1 in the same cancer tissue. The expression of PAK1 and PAK4 increased gradually with the clinical stages in carcinoma tissues (P < 0.01). PAK1 expression was higher in lymph node positive patients, and PAK4 expression was higher in infiltration into serous layer patients (P < 0.05). PAK1 overexpression group has a higher recurrence/metastasis rate compared with that of the PAK1 low expression group. Follow-up analysis showed that the median progression-free survival time of the PAK1 high expression group was significantly shorter than that of the PAK1 low expression group.
PAK1 and PAK4 expression were associated with colorectal cancer metastasis and infiltration, PAK1 high expression may indicate poor prognosis of colorectal cancer.
p21激活激酶（PAKs）是小GTP酶效应蛋白，在许多基本细胞功能中发挥关键作用，包括细胞骨架重组和细胞运动。PAKs在多种组织中广泛表达，且在多种癌症类型中常过度表达。本研究旨在探讨PAK1和PAK4与结直肠癌临床病理特征之间的关系。
通过TaqMan实时聚合酶链反应、免疫组织化学及临床分析，研究结直肠癌患者中PAK1和PAK4的表达情况。
结直肠癌组织中PAK1和PAK4基因的相对表达水平显著高于正常组织（P<0.01）。在同一癌组织中，PAK4的表达高于PAK1。癌组织中PAK1和PAK4的表达随临床分期逐渐升高（P<0.01）。淋巴结阳性患者中PAK1表达较高，浆膜层浸润患者中PAK4表达较高（P<0.05）。与PAK1低表达组相比，PAK1过表达组的复发/转移率更高。随访分析显示，PAK1高表达组的无进展生存期显著短于PAK1低表达组。
PAK1和PAK4的表达与结直肠癌转移及浸润相关，PAK1高表达可能提示结直肠癌预后不良。
