Regen：用于设计基因组装的程序。
Regen: program for designing gene assembly.
作者信息
Jerala R, Turk V
机构信息
Department of Biochemistry, Jozef Stefan Institute, Ljubljana, Yugoslavia.
出版信息
Nucleic Acids Res. 1988 Mar 11;16(5):1759-66. doi: 10.1093/nar/16.5.1759.
Abstract
One of the main problems in constructing synthetic genes is the incorrect hybridisation between the oligonucleotides. The problem is resolved if the sequence uniquely defines the position of the oligonucleotide in the assembled gene. This can be accomplished through the wise partition of dsDNA sequence in the fragments. We describe a program for use in designing such gene assembly. For a given DNA sequence and the approximate location of oligonucleotide boundary it generates all sets of protruding ends that share the smallest homology.
摘要
构建合成基因的主要问题之一是寡核苷酸之间的错误杂交。如果序列能唯一确定寡核苷酸在组装基因中的位置，那么这个问题就能得到解决。这可以通过将双链DNA序列明智地划分为片段来实现。我们描述了一个用于设计这种基因组装的程序。对于给定的DNA序列和寡核苷酸边界的大致位置，它会生成具有最小同源性的所有突出端集合。
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