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RNA的电子指纹识别

Electronic fingerprinting of RNA.

作者信息

Gegenheimer P

机构信息

Department of Biochemistry, University of Kansas, Lawrence 66045.

出版信息

Nucleic Acids Res. 1988 Mar 11;16(5):1799-800. doi: 10.1093/nar/16.5.1799.

DOI:10.1093/nar/16.5.1799
PMID:2451220
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC338173/
Abstract

Software has been developed to assist RNA fingerprinting analysis. One program generates, from a DNA sequence data file, the oligonucleotides resulting from digestion of an RNA transcript labeled with any specified nucleotide(s). Oligonucleotides are sorted according to their position on the fingerprint. Expected molar yields and products of secondary redigestion are also indicated. A second program facilitates calculation of experimental molar yields of oligonucleotides.

摘要

已开发出软件来辅助RNA指纹分析。一个程序可根据DNA序列数据文件生成由用任何指定核苷酸标记的RNA转录本消化产生的寡核苷酸。寡核苷酸根据它们在指纹上的位置进行排序。还会显示预期的摩尔产量和二次消化的产物。第二个程序便于计算寡核苷酸的实验摩尔产量。

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本文引用的文献

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J Biol Chem. 1983 Jul 10;258(13):8365-73.
2
Structural characterization and in vitro processing of Escherichia coli ribosomal RNA transcripts containing 5- triphosphates, leader sequences, 16 S rRNA, and spacer tRNAs.含有5'-三磷酸、前导序列、16S核糖体RNA和间隔转运RNA的大肠杆菌核糖体RNA转录本的结构表征及体外加工
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