School of Medical Sciences, University of Auckland, Auckland, 1142, New Zealand.
School of Biological Sciences, University of Auckland, Auckland, 1142, New Zealand.
Anal Bioanal Chem. 2021 Apr;413(10):2637-2653. doi: 10.1007/s00216-021-03189-8. Epub 2021 Feb 2.
Matrix-assisted laser desorption/ionisation-imaging mass spectrometry (MALDI-IMS) is now an established imaging modality with particular utility in the study of biological, biomedical and pathological processes. In the first instance, the use of stable isotopically labelled (SIL) compounds in MALDI-IMS has addressed technical barriers to increase the accuracy and versatility of this technique. This has undoubtedly enhanced our ability to interpret the two-dimensional ion intensity distributions produced from biological tissue sections. Furthermore, studies using delivery of SIL compounds to live tissues have begun to decipher cell, tissue and inter-tissue metabolism while maintaining spatial resolution. Here, we review both the technical and biological applications of SIL compounds in MALDI-IMS, before using the uptake and metabolism of glucose in bovine ocular lens tissue to illustrate the current limitations of SIL compound use in MALDI-IMS. Finally, we highlight recent instrumentation advances that may further enhance our ability to use SIL compounds in MALDI-IMS to understand biological and pathological processes. Graphical Abstract.
基质辅助激光解吸/电离成像质谱（MALDI-IMS）现已成为一种成熟的成像方式，特别适用于研究生物、生物医学和病理过程。在最初阶段，在 MALDI-IMS 中使用稳定同位素标记（SIL）化合物解决了提高该技术准确性和多功能性的技术障碍。这无疑增强了我们解释从生物组织切片产生的二维离子强度分布的能力。此外，使用 SIL 化合物递送至活组织的研究已经开始破译细胞、组织和组织间代谢，同时保持空间分辨率。在这里，我们回顾了 SIL 化合物在 MALDI-IMS 中的技术和生物学应用，然后使用牛眼晶状体组织中葡萄糖的摄取和代谢来阐明 SIL 化合物在 MALDI-IMS 中的当前应用限制。最后，我们强调了最近的仪器仪表进展，这些进展可能会进一步增强我们在 MALDI-IMS 中使用 SIL 化合物来理解生物和病理过程的能力。图形摘要。
