MALDI 成像质谱分析中的蛋白质鉴定策略：简要综述。
Protein identification strategies in MALDI imaging mass spectrometry: a brief review.
机构信息
Department of Chemistry, Vanderbilt University, 7330 Stevenson Center, Station B 351822, Nashville, TN 37235, USA; Mass Spectrometry Research Center, Vanderbilt University, 465 21st Ave S #9160, Nashville, TN 37235, USA.
Department of Chemistry, Vanderbilt University, 7330 Stevenson Center, Station B 351822, Nashville, TN 37235, USA; Mass Spectrometry Research Center, Vanderbilt University, 465 21st Ave S #9160, Nashville, TN 37235, USA; Department of Biochemistry, Vanderbilt University, 607 Light Hall, Nashville, TN 37205, USA.
出版信息
Curr Opin Chem Biol. 2019 Feb;48:64-72. doi: 10.1016/j.cbpa.2018.10.023. Epub 2018 Nov 23.
Abstract
Matrix assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) is a powerful technology used to investigate the spatial distributions of thousands of molecules throughout a tissue section from a single experiment. As proteins represent an important group of functional molecules in tissue and cells, the imaging of proteins has been an important point of focus in the development of IMS technologies and methods. Protein identification is crucial for the biological contextualization of molecular imaging data. However, gas-phase fragmentation efficiency of MALDI generated proteins presents significant challenges, making protein identification directly from tissue difficult. This review highlights methods and technologies specifically related to protein identification that have been developed to overcome these challenges in MALDI IMS experiments.
摘要
基质辅助激光解吸/电离（MALDI）成像质谱（IMS）是一种强大的技术，可用于在单个实验中研究组织切片中数千种分子的空间分布。由于蛋白质是组织和细胞中重要的功能分子群体，因此蛋白质的成像一直是 IMS 技术和方法发展的重点。蛋白质鉴定对于分子成像数据的生物学背景化至关重要。然而，MALDI 产生的蛋白质的气相碎裂效率存在很大的挑战，使得直接从组织中进行蛋白质鉴定变得困难。本综述重点介绍了为克服 MALDI IMS 实验中的这些挑战而开发的特定于蛋白质鉴定的方法和技术。
相似文献
1
Protein identification strategies in MALDI imaging mass spectrometry: a brief review.MALDI 成像质谱分析中的蛋白质鉴定策略：简要综述。
Curr Opin Chem Biol. 2019 Feb;48:64-72. doi: 10.1016/j.cbpa.2018.10.023. Epub 2018 Nov 23.
2
Matrix-assisted laser desorption/ionization imaging mass spectrometry.基质辅助激光解吸/电离成像质谱法
Int J Mol Sci. 2010;11(12):5040-55. doi: 10.3390/ijms11125040. Epub 2010 Dec 7.
3
Exploring three-dimensional matrix-assisted laser desorption/ionization imaging mass spectrometry data: three-dimensional spatial segmentation of mouse kidney.探索三维基质辅助激光解吸/电离成像质谱数据：小鼠肾脏的三维空间分割。
Anal Chem. 2012 Jul 17;84(14):6079-87. doi: 10.1021/ac300673y. Epub 2012 Jul 5.
4
Applications of stable isotopes in MALDI imaging: current approaches and an eye on the future.稳定同位素在 MALDI 成像中的应用：当前方法及未来展望。
Anal Bioanal Chem. 2021 Apr;413(10):2637-2653. doi: 10.1007/s00216-021-03189-8. Epub 2021 Feb 2.
5
Review of matrix-assisted laser desorption ionization-imaging mass spectrometry for lipid biochemical histopathology.基质辅助激光解吸电离成像质谱技术在脂质生化组织病理学中的应用综述
J Histochem Cytochem. 2015 Oct;63(10):762-71. doi: 10.1369/0022155415596202. Epub 2015 Jul 24.
6
Direct imaging of single cells and tissue at sub-cellular spatial resolution using transmission geometry MALDI MS.利用透射几何 MALDI MS 对单细胞和组织进行亚细胞空间分辨率的直接成像。
J Mass Spectrom. 2012 Nov;47(11):1473-81. doi: 10.1002/jms.3108.
7
MALDI imaging mass spectrometry for direct tissue analysis: a new frontier for molecular histology.用于直接组织分析的基质辅助激光解吸电离成像质谱：分子组织学的新前沿。
Histochem Cell Biol. 2008 Sep;130(3):421-34. doi: 10.1007/s00418-008-0469-9. Epub 2008 Jul 11.
8
On-tissue protein identification and imaging by MALDI-ion mobility mass spectrometry.利用 MALDI-离子淌度质谱进行组织内蛋白质鉴定和成像。
J Am Soc Mass Spectrom. 2010 Mar;21(3):338-47. doi: 10.1016/j.jasms.2009.09.016. Epub 2009 Sep 29.
9
Simultaneous detection of phosphatidylcholines and glycerolipids using matrix-enhanced surface-assisted laser desorption/ionization-mass spectrometry with sputter-deposited platinum film.使用溅射沉积铂膜的基质增强表面辅助激光解吸/电离质谱法同时检测磷脂酰胆碱和甘油脂。
J Mass Spectrom. 2015 Nov;50(11):1264-9. doi: 10.1002/jms.3700.
10
Next-generation technologies for spatial proteomics: Integrating ultra-high speed MALDI-TOF and high mass resolution MALDI FTICR imaging mass spectrometry for protein analysis.下一代空间蛋白质组学技术：整合超高速基质辅助激光解吸电离飞行时间质谱和高质量分辨率基质辅助激光解吸电离傅里叶变换离子回旋共振成像质谱用于蛋白质分析。
Proteomics. 2016 Jun;16(11-12):1678-89. doi: 10.1002/pmic.201600003. Epub 2016 May 10.
引用本文的文献
1
High-spatial-resolution mass spectrometry imaging of biological tissues using a microfluidic probe.使用微流控探针进行生物组织的高空间分辨率质谱成像。
Nat Protoc. 2025 Jun 12. doi: 10.1038/s41596-025-01188-y.
2
Spatial top-down proteomics for the functional characterization of human kidney.用于人类肾脏功能表征的空间自上而下蛋白质组学
Clin Proteomics. 2025 Mar 5;22(1):9. doi: 10.1186/s12014-025-09531-x.
3
Spatial Proteomics by Parallel Accumulation-Serial Fragmentation Supported MALDI MS/MS Imaging: A First Glance Into Multiplexed and Spatial Peptide Identification.基于平行累积-串联碎裂支持的基质辅助激光解吸/电离质谱/质谱成像的空间蛋白质组学：对多重和空间肽鉴定的初步探索。
Rapid Commun Mass Spectrom. 2025 May 15;39(9):e10006. doi: 10.1002/rcm.10006.
4
Cell-Instructive Biomaterials with Native-Like Biochemical Complexity.具有类天然生化复杂性的细胞指导性生物材料。
Annu Rev Biomed Eng. 2025 May;27(1):185-209. doi: 10.1146/annurev-bioeng-120823-020209. Epub 2025 Jan 28.
5
The burgeoning spatial multi-omics in human gastrointestinal cancers.人类胃肠道癌症中新兴的空间多组学。
PeerJ. 2024 Sep 13;12:e17860. doi: 10.7717/peerj.17860. eCollection 2024.
6
Comparative analysis of phosphorylated proteomes between plerocercoid and adult Spirometra mansoni reveals phosphoproteomic profiles of the medical tapeworm.曼氏迭宫绦虫原头蚴与成虫磷酸化蛋白质组比较分析揭示了医学绦虫的磷酸化蛋白质组图谱。
Parasit Vectors. 2024 Aug 31;17(1):371. doi: 10.1186/s13071-024-06454-8.
7
Mass spectrometry in animal health laboratories: recent history, current applications, and future directions.动物健康实验室中的质谱技术：历史回顾、当前应用和未来方向。
J Vet Diagn Invest. 2024 Nov;36(6):777-789. doi: 10.1177/10406387241270071. Epub 2024 Aug 22.
8
Mass spectrometry imaging for spatially resolved multi-omics molecular mapping.用于空间分辨多组学分子图谱的质谱成像
Npj Imaging. 2024;2(1):20. doi: 10.1038/s44303-024-00025-3. Epub 2024 Jul 17.
9
External Data Systems Enable Enhanced (and Sustainable) Fourier Transform Mass Spectrometry Imaging for Legacy Hybrid Linear Ion Trap-Orbitrap Platforms.外部数据系统助力老旧的线性离子阱-轨道阱混合平台实现增强型（且可持续的）傅里叶变换质谱成像。
J Am Soc Mass Spectrom. 2024 Nov 6;35(11):2690-2698. doi: 10.1021/jasms.4c00145. Epub 2024 Jul 20.
10
Investigating Antiprotozoal Chemotherapies with Novel Proteomic Tools-Chances and Limitations: A Critical Review.运用新型蛋白质组学工具研究抗寄生虫化学疗法：机遇与局限——批判性综述。
Int J Mol Sci. 2024 Jun 24;25(13):6903. doi: 10.3390/ijms25136903.
本文引用的文献
1
A functional chitosan-based hydrogel as a wound dressing and drug delivery system in the treatment of wound healing.一种基于壳聚糖的功能性水凝胶，作为伤口敷料和药物递送系统用于伤口愈合治疗。
RSC Adv. 2018 Feb 16;8(14):7533-7549. doi: 10.1039/c7ra13510f. eCollection 2018 Feb 14.
2
MALDI Imaging Analysis of Neuropeptides in Africanized Honeybee ( Apis mellifera) Brain: Effect of Aggressiveness.非洲化蜜蜂（Apis mellifera）脑内神经肽的 MALDI 成像分析：攻击性的影响。
J Proteome Res. 2018 Jul 6;17(7):2358-2369. doi: 10.1021/acs.jproteome.8b00098. Epub 2018 Jun 1.
3
Region of Interest analysis using mass spectrometry imaging of mitochondrial and sarcomeric proteins in acute cardiac infarction tissue.使用心肌和骨骼肌蛋白的质谱成像对急性心肌梗死组织进行感兴趣区域分析。
Sci Rep. 2018 May 10;8(1):7493. doi: 10.1038/s41598-018-25817-7.
4
Recent advances in matrix-assisted laser desorption/ionisation mass spectrometry imaging (MALDI-MSI) for in situ analysis of endogenous molecules in plants.基质辅助激光解吸/电离质谱成像（MALDI-MSI）在植物内源分子原位分析中的最新进展。
Phytochem Anal. 2018 Jul;29(4):351-364. doi: 10.1002/pca.2759. Epub 2018 Apr 17.
5
Designing hydrogels for controlled drug delivery.设计用于控释给药的水凝胶。
Nat Rev Mater. 2016 Dec;1(12). doi: 10.1038/natrevmats.2016.71. Epub 2016 Oct 18.
6
A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage.一种基于多重质谱的分析方法，用于稳健定量 DNA 损伤反应中的磷酸化信号。
Radiat Res. 2018 May;189(5):505-518. doi: 10.1667/RR14963.1. Epub 2018 Feb 23.
7
Enhanced Ion Transmission Efficiency up to m/ z 24 000 for MALDI Protein Imaging Mass Spectrometry.提高 MALDI 蛋白质成像质谱分析的离子传输效率，最高可达 m/z 24000。
Anal Chem. 2018 Apr 17;90(8):5090-5099. doi: 10.1021/acs.analchem.7b05105. Epub 2018 Apr 2.
8
Multiplexed proteome analysis with neutron-encoded stable isotope labeling in cells and mice.细胞和小鼠中基于中子编码稳定同位素标记的多重蛋白质组分析。
Nat Protoc. 2018 Jan;13(1):293-306. doi: 10.1038/nprot.2017.121. Epub 2018 Jan 11.
9
Anatomical-Molecular Distribution of EphrinA1 in Infarcted Mouse Heart Using MALDI Mass Spectrometry Imaging.利用 MALDI 质谱成像技术研究 EphrinA1 在缺血性损伤的小鼠心脏中的解剖-分子分布
J Am Soc Mass Spectrom. 2018 Mar;29(3):527-534. doi: 10.1007/s13361-017-1869-7. Epub 2018 Jan 5.
10
High-throughput double emulsion-based microfluidic production of hydrogel microspheres with tunable chemical functionalities toward biomolecular conjugation.基于高通量双重乳液的微流控法制备具有可调化学功能的水凝胶微球，用于生物分子偶联。
Lab Chip. 2018 Jan 16;18(2):323-334. doi: 10.1039/c7lc01088e.
Zotero 插件