Goldberg David J
Icahn School of Medicine, New York, NY, USA.
J Cosmet Dermatol. 2021 Apr;20(4):1104-1109. doi: 10.1111/jocd.13970. Epub 2021 Feb 8.
Radiofrequency (RF) is commonly recognized treatment option for fat reduction, utilizing heat-induced adipocyte deletion. HIFEM treatment has been proven to be an effective tool for body shaping.
To document the structural changes in human subcutaneous tissue induced by the combination of RF treatment with the HIFEM procedure.
Four subjects (51.50 ± 6.35 years, 22.59 ± 3.21 kg/m ) received three 30-minute abdominal treatments consisting of RF therapy and HIFEM. One subject (57 years, 23.60 kg/m ) served as a control. Punch biopsies were collected at baseline, 1-week, and 1-month post-treatment. Samples were sliced and stained with H&E. Waist circumference, digital photographs, satisfaction, and therapy comfort were assessed. Subjects were monitored for any adverse event, and fat temperatures were measured.
Baseline samples showed a healthy appearance of adipocytes, composed of round-shaped unilocular cells of uniform size. At follow-up, treated adipocytes demonstrated nuclear and shape changes with consequent fat reduction. Adipocytes were found to be flattened/shrunken and of smaller size (-33.5% at 1 week; -31.7% at 1-month) along with occasional ruptures of the cytoplasmic membrane. In contrast to baseline, pyknotic nuclei with condensed nuclear chromatin were seen at 1-week and 1-month post-treatment. The control samples showed no treatment-related changes. Waist circumference decreased by an average of 2.20 cm in the treated patients. No adverse events were observed. The fat temperature reached 42-45°C, during treatment; the therapy was comfortable with high patient satisfaction.
Results suggest the efficacy and safety of the therapy combining RF and HIFEM. The adipocyte deletion and shrinkage resulted in overall reduction of fat tissue.
射频（RF）是一种公认的减脂治疗方法，通过热诱导使脂肪细胞凋亡。高强度聚焦电磁（HIFEM）治疗已被证明是一种有效的塑形工具。
记录射频治疗与HIFEM程序联合应用引起的人体皮下组织的结构变化。
4名受试者（年龄51.50±6.35岁，体重指数22.59±3.21kg/m²）接受了三次30分钟的腹部治疗，包括射频治疗和HIFEM。1名受试者（年龄57岁，体重指数23.60kg/m²）作为对照。在基线、治疗后1周和1个月时采集打孔活检样本。样本切片后用苏木精和伊红染色。评估腰围、数码照片、满意度和治疗舒适度。监测受试者有无不良事件，并测量脂肪温度。
基线样本显示脂肪细胞外观健康，由大小均匀的圆形单泡状细胞组成。随访时，经治疗的脂肪细胞出现核及形态变化，随后脂肪减少。脂肪细胞扁平/缩小，体积变小（1周时减少33.5%；1个月时减少31.7%），偶尔可见细胞质膜破裂。与基线相比，治疗后1周和1个月时可见核染色质浓缩的固缩核。对照样本未显示与治疗相关的变化。治疗患者的腰围平均减少2.20cm。未观察到不良事件。治疗期间脂肪温度达到42-45°C；该治疗舒适，患者满意度高。
结果表明射频与HIFEM联合治疗的有效性和安全性。脂肪细胞的凋亡和萎缩导致脂肪组织整体减少。
