Yang Rui, He Xiuquan, Niu Guangle, Meng Fangfang, Lu Qing, Liu Zhiqiang, Yu Xiaoqiang
State Key Laboratory of Crystal Materials, and Advanced Medical Research Institute, Shandong University, Jinan 250100, P. R. China.
Department of Anatomy, School of Basic Medical Sciences, Shandong University, Jinan 250012, P. R. China.
ACS Sens. 2021 Apr 23;6(4):1552-1559. doi: 10.1021/acssensors.0c02372. Epub 2021 Feb 3.
Subcellular organelles play indispensable roles in diverse biological processes by their precise mutual cooperation. Thus, the development of a single fluorescent probe (SF-probe) for simultaneous and discriminable visualization of different organelles and their dynamics during certain bioprocess is significant, yet remains greatly challenging. Herein, for the first time, we rationally prepared a pH-sensitive SF-probe (named HMBI) for the simultaneous two-color visualization of nuclei and mitochondria and monitoring cell apoptosis. HMBI shows remarkable ratiometric fluorescence changes toward pH changes. Due to different pH environments in subcellular organelles, HMBI can image nuclei and mitochondria with green and red emission, respectively. HMBI can monitor drug-induced cell apoptosis with dramatically decreased red emission in mitochondria but almost unchanged green emission in nuclei, and the shrinking and pyknotic nuclei are also observed during cell apoptosis. HMBI possesses tremendous potential in two-color biomedical imaging of the dynamic changes of nuclei and mitochondria in many physiological processes.
亚细胞细胞器通过精确的相互协作在多种生物学过程中发挥着不可或缺的作用。因此，开发一种单一荧光探针（SF-探针）用于在特定生物过程中同时且可区分地可视化不同细胞器及其动态变化具有重要意义，但仍然极具挑战性。在此，我们首次合理制备了一种pH敏感的SF-探针（命名为HMBI），用于细胞核和线粒体的双色同时可视化以及监测细胞凋亡。HMBI对pH变化表现出显著的比率荧光变化。由于亚细胞细胞器中不同的pH环境，HMBI能够分别以绿色和红色发射对细胞核和线粒体进行成像。HMBI可以监测药物诱导的细胞凋亡，线粒体中的红色发射显著降低，而细胞核中的绿色发射几乎不变，并且在细胞凋亡过程中还观察到细胞核的收缩和固缩。HMBI在许多生理过程中对细胞核和线粒体动态变化的双色生物医学成像方面具有巨大潜力。
