用于在亚细胞水平跟踪药物动态的先进成像技术。
Advanced imaging techniques for tracking drug dynamics at the subcellular level.
机构信息
Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA.
Department of Chemistry, University of Cincinnati, Cincinnati, OH 45221, USA.
出版信息
Adv Drug Deliv Rev. 2023 Aug;199:114978. doi: 10.1016/j.addr.2023.114978. Epub 2023 Jun 28.
Abstract
Optical microscopes are an important imaging tool that have effectively advanced the development of modern biomedicine. In recent years, super-resolution microscopy (SRM) has become one of the most popular techniques in the life sciences, especially in the field of living cell imaging. SRM has been used to solve many problems in basic biological research and has great potential in clinical application. In particular, the use of SRM to study drug delivery and kinetics at the subcellular level enables researchers to better study drugs' mechanisms of action and to assess the efficacy of their targets in vivo. The purpose of this paper is to review the recent advances in SRM and to highlight some of its applications in assessing subcellular drug dynamics.
摘要
光学显微镜是一种重要的成像工具，它有效地推动了现代生物医学的发展。近年来，超分辨率显微镜（SRM）已成为生命科学领域中最受欢迎的技术之一，特别是在活细胞成像领域。SRM 已被用于解决基础生物学研究中的许多问题，并且在临床应用中具有很大的潜力。特别是，使用 SRM 来研究亚细胞水平的药物传递和动力学，使研究人员能够更好地研究药物的作用机制，并评估其在体内的靶标疗效。本文旨在综述 SRM 的最新进展，并强调其在评估亚细胞药物动力学方面的一些应用。
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Nat Chem Biol. 2023 Dec;19(12):1448-1457. doi: 10.1038/s41589-023-01364-9. Epub 2023 Jun 15.
2
Monitoring lysosomal acidity.
Nat Chem Biol. 2023 Dec;19(12):1434-1435. doi: 10.1038/s41589-023-01348-9.
3
An ER-targeted "reserve-release" fluorogen for topological quantification of reticulophagy.
Biomaterials. 2023 Jan;292:121929. doi: 10.1016/j.biomaterials.2022.121929. Epub 2022 Nov 22.
4
NanoSIMS combined with fluorescence microscopy as a tool for subcellular imaging of isotopically labeled platinum-based anticancer drugs.
Chem Sci. 2014 Jun 6;5(8):3135-3143. doi: 10.1039/c3sc53426j. eCollection 2014 Jun 30.
5
Neuronal activity induces glucosylceramide that is secreted via exosomes for lysosomal degradation in glia.
Sci Adv. 2022 Jul 15;8(28):eabn3326. doi: 10.1126/sciadv.abn3326. Epub 2022 Jul 13.
6
Super-resolution analyzing spatial organization of lysosomes with an organic fluorescent probe.
Exploration (Beijing). 2022 Jun;2(3). doi: 10.1002/exp.20210215. Epub 2022 Mar 22.
7
Organelle Interaction and Drug Discovery: Towards Correlative Nanoscopy and Molecular Dynamics Simulation.
Front Pharmacol. 2022 Jun 20;13:935898. doi: 10.3389/fphar.2022.935898. eCollection 2022.
8
Single Image Capture of Bioactive Ion Crosstalk within Inter-Organelle Membrane Contacts at Nanometer Resolution.
Small Methods. 2022 Aug;6(8):e2200321. doi: 10.1002/smtd.202200321. Epub 2022 Jul 1.
9
De Novo Design of A Membrane-Anchored Probe for Multidimensional Quantification of Endocytic Dynamics.
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10
Long-term live-cell lipid droplet-targeted biosensor development for nanoscopic tracking of lipid droplet-mitochondria contact sites.
Theranostics. 2021 Jun 22;11(16):7767-7778. doi: 10.7150/thno.59848. eCollection 2021.
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