Department of Plant Production and Genetics, Faculty of Agriculture, University of Zanjan, Zanjan, Iran.
Department of Pharmaceutical Biomaterials, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.
Nat Prod Res. 2022 Mar;36(5):1385-1390. doi: 10.1080/14786419.2021.1875463. Epub 2021 Feb 3.
The medicinal plant, , is used for the treatment of several diseases in North-western of Iran. The purpose of this study was to investigate the phytochemical content, cytotoxicity assay against SW-480 and HCT-116 cells and anti-haemolytic activities using HPLC-PDA, GC/MS, MTT and spectrophotometry methods, respectively. Phytochemical screening revealed the presence of different terpenoids and flavonoids such as baicalin, tricin and wogonin and also some of the other medicinally active compounds such as conhydrine and cannabidiol. MTT results revealed that HCT-116 cells were more sensitive to examined extracts compared with SW-480 cells. Methanol extract had the most anti-proliferative activity against HCT-116 and SW-480 cells at 48 h with IC50 values of 614.5 and 592.3 µg/mL, respectively. Also, all samples had no significant haemolytic effect on human erythrocytes. Our results revealed that root extract has a promising anticancer activity, indicating the presence of major anti-cancer agents on human colon cell lines.
药用植物 被用于治疗伊朗西北部的几种疾病。本研究的目的是分别采用 HPLC-PDA、GC/MS、MTT 和分光光度法，研究其植物化学成分、对 SW-480 和 HCT-116 细胞的细胞毒性以及抗溶血活性。植物化学筛选显示存在不同的萜类化合物和类黄酮，如黄芩苷、白杨素和甘草素，以及一些其他具有药用活性的化合物，如盐酸小檗碱和大麻二酚。MTT 结果表明，与 SW-480 细胞相比，HCT-116 细胞对所检测的提取物更敏感。甲醇提取物对 HCT-116 和 SW-480 细胞的增殖活性最强，在 48 小时时的 IC50 值分别为 614.5 和 592.3μg/mL。此外，所有样品对人红细胞均无显著溶血作用。我们的研究结果表明， 根提取物具有良好的抗癌活性，提示其在人结肠细胞系中存在主要的抗癌成分。
