Department of Plant production and Genetics, Faculty of Agriculture, University of Zanjan, Zanjan, Iran.
Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran.
Nat Prod Res. 2022 Aug;36(15):4031-4035. doi: 10.1080/14786419.2021.1903003. Epub 2021 Mar 25.
is a well-known ethnobotanical herb for the treatment of various diseases in Iranian traditional medicine. In this study, dichloromethanolic fraction of partitioned methanol root and shoot extract was analysed by silica gel column chromatography, thin layer chromatography and high-performance liquid chromatography combined with photodiode-array detector, and coupled to electrospray ionization with Q-Exactive Orbitrap mass spectrometry (HPLC-PDA-ESI-MS) in positive ion mode. Metabolites were tentatively characterized by comparing their mass spectrometry spectra with those of bibliographic data. A total of 11 flavonoids were identified from different fractions. Results showed that root and shoot produced very similar flavone patterns characterized by the presence of baicalein, tricin, tenaxin I, tenaxin II, skullcapflavon II, chrysin, wogonin and isorhamnetin. Norwogonin and apigenin-7-glucoside were identified only from shoot. Remarkably, Tenaxin I, II, tricin, apigenin-7-glucoside and norwogonin were tentatively profiled as new compounds for the first time from formula.
是一种在伊朗传统医学中用于治疗各种疾病的著名民族植物药。在这项研究中，采用硅胶柱色谱、薄层色谱和高效液相色谱-光电二极管阵列检测器（HPLC-PDA）结合电喷雾电离-轨道阱高分辨质谱（HPLC-PDA-ESI-MS）对甲醇萃取物的根和茎的二氯甲烷部分进行了分析。通过将质谱图谱与文献数据进行比较，对代谢产物进行了初步表征。从不同部分鉴定出了 11 种黄酮类化合物。结果表明，根和茎产生的黄酮类化合物模式非常相似，特征为黄芩素、白杨素、千里光宁 I、千里光宁 II、黄芩素 II、白杨素、高车前素和异鼠李素。水飞蓟宾和芹菜素-7-葡萄糖苷仅从茎中鉴定出来。值得注意的是，千里光宁 I、II、白杨素、芹菜素-7-葡萄糖苷和水飞蓟宾被初步确认为新化合物，这是它们首次从 中得到描述。
