Science Faculty, Department of Biology, Selcuk University, Konya, Turkey.
Department of Physical and Analytical Chemistry, University of Jaén, Jaén, Spain.
J Pharm Pharmacol. 2019 Feb;71(2):270-280. doi: 10.1111/jphp.13030. Epub 2018 Oct 10.
This study investigates into the biological effects of solvent extracts (ethyl acetate, methanol, and water) of Scutellaria orientalis L. and Scutellaria salviifolia Benth. based on its enzyme inhibitory activity and antioxidant ability together with the screening of bioactive compounds.
Total and individual bioactive components were determined using spectrophotometric and HPLC-ESI-MS methods. Six antioxidant assays were conducted and enzyme inhibition was tested against key enzymes linked to the pathology of common chronic disorders.
Results revealed that the aqueous extracts of both plants exerted better 2,2'-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid scavenging, reducing power, metal chelating, and α-glucosidase inhibitory activities. The methanol extracts showed highest tyrosinase inhibition and antioxidant activity in phosphomolybdenum assay while the less polar ethyl acetate extracts showed better acetylcholinesterase, butyrylcholinesterase, and α-amylase inhibition. Phytochemical evaluation by HPLC-ESI-MS revealed the presence of high amounts of flavones.
Scutellaria orientalis and S. salviifolia are important sources of bioactive agents that warrants further studies.
本研究基于酶抑制活性和抗氧化能力，以及生物活性化合物的筛选，考察了黄芩和丹参的溶剂提取物（乙酸乙酯、甲醇和水）的生物学效应。
采用分光光度法和 HPLC-ESI-MS 法测定总生物活性成分和各生物活性成分。进行了 6 种抗氧化测定，并对与常见慢性疾病病理相关的关键酶进行了酶抑制测试。
结果表明，两种植物的水提物对 2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）具有更好的清除作用、还原能力、金属螯合能力和α-葡萄糖苷酶抑制活性。甲醇提取物在磷钼酸盐测定中显示出最高的酪氨酸酶抑制作用和抗氧化活性，而疏水性较弱的乙酸乙酯提取物对乙酰胆碱酯酶、丁酰胆碱酯酶和α-淀粉酶的抑制作用更好。HPLC-ESI-MS 的植物化学评价表明，黄酮类化合物的含量很高。
黄芩和丹参是生物活性物质的重要来源，值得进一步研究。
