Ulrich F
Surgical Research Unit, Boston Veterans Administration Medical Center, Massachusets 02130.
Proc Soc Exp Biol Med. 1988 Apr;187(4):488-92. doi: 10.3181/00379727-187-42693.
When Raji cells were cultured for 3 days in serum-free medium, addition of 2-deoxy-D-ribose at the start of culture inhibited incorporation of [3H]thymidine and cell division. At deoxyribose concentrations between 1 and 5 mM, viability was 80% or greater after 3 days of culture even though 5 mM deoxyribose inhibited thymidine incorporation 95-99%. Inhibition by deoxyribose could be completely reversed if the culture medium was replaced with fresh medium up to 8 hr after the start of culture. The inhibition was specific for deoxyribose since other monosaccharides had no effect. Inhibition of DNA synthesis did not appear to be due to depletion of essential nutrients in the medium since the percentage inhibition of thymidine incorporation by cells cultured either in suboptimal serum-free media or in media supplemented with 0.025-5% human AB serum was similar. When DNA repair synthesis was measured as hydroxyurea-resistant thymidine incorporation, addition of deoxyribose to Raji cultures caused increased thymidine incorporation. These results, together with data from others, suggest that deoxyribose damages DNA.
当Raji细胞在无血清培养基中培养3天时，在培养开始时添加2-脱氧-D-核糖会抑制[3H]胸苷的掺入和细胞分裂。在脱氧核糖浓度为1至5 mM时，培养3天后细胞活力仍为80%或更高，尽管5 mM脱氧核糖会抑制胸苷掺入95 - 99%。如果在培养开始后8小时内用新鲜培养基替换培养基，脱氧核糖的抑制作用可以完全逆转。这种抑制作用对脱氧核糖具有特异性，因为其他单糖没有影响。DNA合成的抑制似乎不是由于培养基中必需营养物质的耗尽，因为在次优无血清培养基或补充有0.025 - 5%人AB血清的培养基中培养的细胞对胸苷掺入的抑制百分比相似。当将DNA修复合成测定为耐羟基脲的胸苷掺入时，向Raji培养物中添加脱氧核糖会导致胸苷掺入增加。这些结果与其他研究数据一起表明，脱氧核糖会损伤DNA。
