从骨标本中分离并制备符合药品生产质量管理规范（GMP）级别的骨髓基质细胞
The Isolation and Manufacture of GMP-Grade Bone Marrow Stromal Cells from Bone Specimens.
作者信息
Dias Rhayra B, Bonfim Danielle C
机构信息
Research Division, National Institute of Traumatology and Orthopedics, Rio de Janeiro, Brazil.
Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
出版信息
Methods Mol Biol. 2021;2286:95-105. doi: 10.1007/7651_2020_319.
Bone marrow stromal cells (BMSCs, also known as bone marrow mesenchymal stem cells) are a plastic-adherent heterogeneous cell population that contain inherent skeletal progenitors and a subset of multipotential skeletal stem cells (SSCs). Application of BMSCs in therapeutic protocols implies its isolation and expansion under good manufacturing practices (GMP). Here we describe the procedures we have found to successfully generate practical BMSCs numbers, with preserved biological potency.
骨髓基质细胞（BMSCs，也称为骨髓间充质干细胞）是一类贴壁生长的异质性细胞群体，其中包含内在的骨骼祖细胞和一部分多能骨骼干细胞（SSCs）。在治疗方案中应用BMSCs意味着要在良好生产规范（GMP）下对其进行分离和扩增。在此，我们描述了已发现的能成功产生数量可观且生物学活性得以保留的BMSCs的方法。
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