Wang Jiasi, Staheli Jeannette P, Wu Andrew, Kreutz Jason E, Hu Qiongzheng, Wang Jingang, Schneider Thomas, Fujimoto Bryant S, Qin Yuling, Yen Gloria S, Weng Bob, Shibley Kara, Haynes Halia, Winer Rachel L, Feng Qinghua, Chiu Daniel T
Department of Chemistry, University of Washington, Seattle, Washington 98195, United States.
Center for Global Infectious Disease Research, Seattle Children's Research Institute, Seattle, Washington 98105, United States.
Anal Chem. 2021 Feb 16;93(6):3266-3272. doi: 10.1021/acs.analchem.0c04973. Epub 2021 Feb 3.
Cervical cancer is the fourth-leading cause of cancer deaths among women worldwide and most cases occur in developing countries. Detection of high-risk (HR) HPV, the etiologic agent of cervical cancer, is a primary screening method for cervical cancer. However, the current gold standard for HPV detection, real-time PCR, is expensive, time-consuming, and instrumentation-intensive. A rapid, low-cost HPV detection method is needed for cervical cancer screening in low-resource settings. We previously developed a digital loop-mediated isothermal amplification (dLAMP) assay for rapid, quantitative detection of nucleic acids without the need for thermocycling. This assay employs a microfluidic self-digitization chip to automatically digitize a sample into an array of nanoliter wells in a simple assay format. Here we evaluate the dLAMP assay and self-digitization chip for detection of the commonly tested 14 high-risk HPVs in clinical samples. The dLAMP platform provided reliable genotyping and quantitative detection of the 14 high-risk HPVs with high sensitivity, demonstrating its potential for simple, rapid, and low-cost diagnosis of HPV infection.
宫颈癌是全球女性癌症死亡的第四大原因，且大多数病例发生在发展中国家。高危型（HR）人乳头瘤病毒（HPV）是宫颈癌的致病因子，检测该病毒是宫颈癌的主要筛查方法。然而，目前HPV检测的金标准——实时聚合酶链反应（PCR），成本高、耗时且需要大量仪器设备。在资源匮乏地区进行宫颈癌筛查需要一种快速、低成本的HPV检测方法。我们之前开发了一种数字环介导等温扩增（dLAMP）检测法，用于快速、定量检测核酸，无需热循环。该检测法采用微流控自数字化芯片，以简单的检测形式将样品自动数字化成一系列纳升孔。在此，我们评估dLAMP检测法和自数字化芯片在临床样本中检测常见的14种高危型HPV的性能。dLAMP平台对14种高危型HPV提供了可靠的基因分型和定量检测，灵敏度高，证明了其在HPV感染的简单、快速和低成本诊断方面的潜力。
