过氧甲酸预处理甘蔗渣的酶解强化和氢气生成。
Enhanced enzymatic hydrolysis and hydrogen production of sugarcane bagasse pretreated by peroxyformic acid.
机构信息
Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China.
The Key Laboratory of Biological Resources and Ecology of Pamirs Plateau in Xinjiang Uygur Autonomous Region, The Key Laboratory of Ecology and Biological Resources in Yarkand Oasis at Colleges & Universities under the Department of Education of Xinjiang Uygur Autonomous Region, College of Life and Geographic Sciences, Kashi University, Kashi, China.
出版信息
Bioresour Technol. 2021 Apr;326:124751. doi: 10.1016/j.biortech.2021.124751. Epub 2021 Jan 27.
Pretreatment plays a key role in biofuel production from lignocellulosic biomass. In this study, the main factors of peroxyformic acid (PA) pretreatment were optimized in the light of enzymolysis efficiency and composition analysis of pretreated sugarcane bagasse (SCB). Lignin was significantly removed (59.0%) and a complete saccharification level (103.6%) was obtained for the pretreated SCB with slight cellulose loss (9.2%) under the optimized pretreatment conditions. The effects of PA pretreatment on the structural characteristics of SCB were also studied and the digestibility of pretreated SCB was also evaluated by dark fermentative hydrogen production with an enriched anaerobic cellulolytic microbial consortium MC1. The hydrogen production increased by 195.5% (based on initial SCB) and the abundance of dominant hemicellulose-degradation genus Thermoanaerobacterium increased from 23.8% to 40.2% due to the remaining and accessible hemicellulose in PA pretreated SCB.
预处理在木质纤维素生物质生物燃料生产中起着关键作用。在这项研究中，根据酶解效率和预处理甘蔗渣（SCB）的成分分析，优化了过氧甲酸（PA）预处理的主要因素。在优化的预处理条件下，SCB 的木质素去除率达到 59.0%，且保留了 9.2%的纤维素，同时获得了完全的糖化水平（103.6%）。还研究了 PA 预处理对 SCB 结构特征的影响，并通过富集厌氧纤维素分解微生物群落 MC1 的暗发酵产氢来评估预处理 SCB 的可消化性。由于 PA 预处理 SCB 中仍有和可利用的半纤维素，产氢量增加了 195.5%（基于初始 SCB），优势产氢菌 Thermoanaerobacterium 的丰度从 23.8%增加到 40.2%。
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