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粪便中 microRNA-16-5p 和 microRNA-21-5p 的鉴定作为炎症性肠病潜在的非侵入性生物标志物。

Identification of microRNA-16-5p and microRNA-21-5p in feces as potential noninvasive biomarkers for inflammatory bowel disease.

机构信息

Department of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.

Hubei Clinical Center and Key Laboratory of Intestinal and Colorectal Diseases, Wuhan 430071, China.

出版信息

Aging (Albany NY). 2021 Feb 1;13(3):4634-4646. doi: 10.18632/aging.202428.

DOI:10.18632/aging.202428
PMID:33535181
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7906140/
Abstract

BACKGROUND

Inflammatory bowel disease (IBD) is a chronic idiopathic gastrointestinal disease. Increasing evidence suggests that microRNAs (miRNAs) may participate in the pathophysiology of IBD.

METHODS

A miRCURY™ LNA Array and hybridization were employed to screen for differentially expressed miRNAs (DEMs) in fecal specimens from 41 IBD patients (22 ulcerative colitis (UC), 19 Crohn's disease (CD)) and 23 healthy controls (HC). RT-qPCR was performed to confirm the findings. The DEMs target genes and corresponding biological functions were predicted by bioinformatics analysis.

RESULTS

Compared with HC, miR-16-5p in the feces was up-regulated both in UC and CD patients (p < 0.01), while miR-21-5p was up-regulated only in UC patients (p < 0.01). TargetScan 7.2, miRWalk, and miRDB were used to predict 216 public target genes of miR-16-5p and miR-21-5p, and six hub genes (PIK3R1, GRB2, SUZ12, NTRK2, Smurf2, and WWP1) were analyzed using the STRING database and Cytoscape. All the hub genes promote the occurrence and development of IBD-related colorectal cancer.

CONCLUSIONS

The elevated levels of miR-16-5p and miR-21-5p in feces of IBD patients have to guide significance for the noninvasive clinical diagnosis of IBD and have a warning effect on the occurrence of IBD-related colorectal cancer.

摘要

背景

炎症性肠病（IBD）是一种慢性特发性胃肠道疾病。越来越多的证据表明，微小 RNA（miRNA）可能参与 IBD 的病理生理学过程。

方法

采用 miRCURY™ LNA 阵列和杂交技术筛选 41 例 IBD 患者（22 例溃疡性结肠炎（UC），19 例克罗恩病（CD））和 23 例健康对照（HC）粪便标本中的差异表达 miRNA（DEM）。采用 RT-qPCR 验证发现。通过生物信息学分析预测 DEMs 的靶基因及其相应的生物学功能。

结果

与 HC 相比，UC 和 CD 患者粪便中 miR-16-5p 均上调（p<0.01），而 miR-21-5p 仅在 UC 患者中上调（p<0.01）。TargetScan 7.2、miRWalk 和 miRDB 用于预测 miR-16-5p 和 miR-21-5p 的 216 个公共靶基因，使用 STRING 数据库和 Cytoscape 分析六个枢纽基因（PIK3R1、GRB2、SUZ12、NTRK2、Smurf2 和 WWP1）。所有枢纽基因均促进 IBD 相关结直肠癌的发生和发展。

结论

IBD 患者粪便中 miR-16-5p 和 miR-21-5p 水平升高对 IBD 的非侵入性临床诊断具有重要指导意义，并对 IBD 相关结直肠癌的发生具有警示作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4162/7906140/679f8d5af2dc/aging-13-202428-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4162/7906140/0217db343c00/aging-13-202428-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4162/7906140/679f8d5af2dc/aging-13-202428-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4162/7906140/0217db343c00/aging-13-202428-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4162/7906140/679f8d5af2dc/aging-13-202428-g002.jpg

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