Zhang Yanru, Xu Rui, Xiang Yinping, Lu Yue, Jia Meiying, Huang Jing, Xu Zhengyong, Cao Jiao, Xiong Weiping, Yang Zhaohui
College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.
Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Guangdong Institute of Eco-environmental Science Technology, Guangzhou 510650, PR China; National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangzhou 510650, PR China.
J Hazard Mater. 2021 Mar 5;405:124206. doi: 10.1016/j.jhazmat.2020.124206. Epub 2020 Oct 8.
This study explored the fate of mobile genetic elements (MGEs) in anaerobic digestion (AD) system with four nanoparticles (NPs) added, including carbon NPs, AlO NPs, ZnO NPs, and CuO NPs. 16S rRNA amplicon sequencing and quantitative PCR to investigate the microbial community, MGEs abundance and the potential host in the AD process. The results of high-throughput sequencing showed that ZnO NPs and CuO NPs significantly reduced the microbial diversity and significantly changed the microbial community structure. Simultaneously, the absolute abundance of MGEs increased by 145.01%, 159.67%, 354.70%, and 132.80% on the carbon NPs, AlO NPs, ZnO NPs, and CuO NPs. The enrichment rate of tnpA-03 in ZnO NPs group was the highest, which could reach up to 2854.80%. Co-occurrence analysis revealed that Proteobacteria harbored the vast majority of MGEs followed by Firmicutes. Redundancy analysis and variation partitioning analysis showed that metabolites were the main factors that shifted the succession of bacterial communities. Moreover, there were significant positive correlations between metabolites and part MGEs (such as tnpA-01, tnpA-02, tnpA-03, tnpA-04, tnpA-05, tnpA-07 and ISCR1). This study provides a new perspective that NPs increase the risk of antibiotic resistance through MGEs during AD process.
本研究探讨了在添加了四种纳米颗粒（NPs）的厌氧消化（AD）系统中移动遗传元件（MGEs）的命运，这四种纳米颗粒包括碳纳米颗粒、氧化铝纳米颗粒、氧化锌纳米颗粒和氧化铜纳米颗粒。采用16S rRNA扩增子测序和定量PCR来研究AD过程中的微生物群落、MGEs丰度及潜在宿主。高通量测序结果表明，氧化锌纳米颗粒和氧化铜纳米颗粒显著降低了微生物多样性，并显著改变了微生物群落结构。同时，碳纳米颗粒、氧化铝纳米颗粒、氧化锌纳米颗粒和氧化铜纳米颗粒上MGEs的绝对丰度分别增加了145.01%、159.67%、354.70%和132.80%。氧化锌纳米颗粒组中tnpA-03的富集率最高，可达2854.80%。共现分析表明，变形菌门携带了绝大多数的MGEs，其次是厚壁菌门。冗余分析和变异分配分析表明，代谢产物是导致细菌群落演替变化的主要因素。此外，代谢产物与部分MGEs（如tnpA-01、tnpA-02、tnpA-03、tnpA-04、tnpA-05、tnpA-07和ISCR1）之间存在显著的正相关。本研究提供了一个新的视角，即纳米颗粒在AD过程中通过MGEs增加了抗生素耐药性的风险。
