College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.
College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.
Bioresour Technol. 2020 May;304:123016. doi: 10.1016/j.biortech.2020.123016. Epub 2020 Feb 18.
The effects of four conductive nanomaterials (nano-carbon powder, nano-AlO, nano-ZnO, nano-CuO) on sludge anaerobic digestion (AD) performance and microbial community were investigated through a 36-day fermentation experiment. Results showed that biogas production enhanced by 16.9% and 23.4% with nano-carbon powder and nano-AlO added but decreased by 90.2% and 17.3% with nano-ZnO and nano-CuO. Total solids (TS) removal efficiency was increased by 38.73% and 27.11% with nano-carbon powder and nano-AlO added but decreased by 70.67% and 43.70% with nano-ZnO and nano-CuO. Kinetic analysis indicated four conductive nanomaterials could shorten the lag phase of AD sludge with an average rate of 51.75%. 16S rRNA amplicon sequencing results demonstrated microbes such as Syntrophomonas and Methanosaeta were enriched in nano-carbon powder and nano-AlO reactors. However, microbial community diversity and richness were both inhibited by adding nano-ZnO and nano-CuO. Redundancy analysis (RDA) revealed that genera belong to Firmicutes and Chloroflexi could conduce to methanogenesis process.
采用 36 天发酵实验，研究了四种导电纳米材料（纳米碳粉、纳米 Al2O3、纳米 ZnO、纳米 CuO）对污泥厌氧消化（AD）性能和微生物群落的影响。结果表明，添加纳米碳粉和纳米 Al2O3 可分别使沼气产量提高 16.9%和 23.4%，而添加纳米 ZnO 和纳米 CuO 则分别使沼气产量降低 90.2%和 17.3%。添加纳米碳粉和纳米 Al2O3 可使总固体（TS）去除效率分别提高 38.73%和 27.11%，而添加纳米 ZnO 和纳米 CuO 则使 TS 去除效率分别降低 70.67%和 43.70%。动力学分析表明，四种导电纳米材料均可使 AD 污泥的迟滞期平均缩短 51.75%。16S rRNA 扩增子测序结果表明，纳米碳粉和纳米 Al2O3 反应器中富集了产甲烷菌（如互营单胞菌属和产甲烷丝菌属）。然而，添加纳米 ZnO 和纳米 CuO 会抑制微生物群落的多样性和丰富度。冗余分析（RDA）表明，厚壁菌门和绿弯菌门的属与产甲烷过程有关。
