Yang Yuran, Zhang Can, Li Kaili, Li Zhenlun
Chongqing Key Laboratory of Soil Multiscale Interfacial Process, College of Resources and Environment, Southwest University, Chongqing 400716, China.
School of Chemical Engineering, University of Queensland, Brisbane, QLD 4072, Australia.
Microorganisms. 2021 Oct 20;9(11):2189. doi: 10.3390/microorganisms9112189.
The negative effect of ZnO nanoparticles (ZnO-NPs) on the biological removal of nitrate (NO) has received extensive attention, but the underlying mechanism is controversial. Additionally, there is no research on Fe used to alleviate the cytotoxicity of NPs. In this paper, the effects of different doses of ZnO-NPs on the growth and NO removal of Y-11 were studied with or without Fe. The results showed that ZnO-NPs had a dose-dependent inhibition on the growth and NO removal of Y-11 and achieved cytotoxic effects through both the NPs themselves and the released Zn. The addition of Fe changed the behavior of ZnO-NPs in an aqueous solution (inhibiting the release of toxic Zn and promoting the aggregation of ZnO-NPs), thereby alleviating the poisonous effect of ZnO-NPs on the growth and nitrogen removal of Y-11. This study provides a theoretical method for exploring the mitigation of the acute toxicity of ZnO-NPs to denitrifying microorganisms.
氧化锌纳米颗粒（ZnO-NPs）对硝酸盐（NO）生物去除的负面影响已受到广泛关注，但其潜在机制仍存在争议。此外，关于使用铁来减轻纳米颗粒细胞毒性的研究尚无报道。本文研究了不同剂量的ZnO-NPs在有无铁存在的情况下对Y-11生长和NO去除的影响。结果表明，ZnO-NPs对Y-11的生长和NO去除具有剂量依赖性抑制作用，并通过纳米颗粒本身和释放的锌实现细胞毒性作用。铁的添加改变了ZnO-NPs在水溶液中的行为（抑制有毒锌的释放并促进ZnO-NPs的聚集），从而减轻了ZnO-NPs对Y-11生长和脱氮的毒害作用。本研究为探索减轻ZnO-NPs对反硝化微生物急性毒性提供了理论方法。
