Alors David, Boussiba Sammy, Zarka Aliza
Microalgal Biotechnology Laboratory, the Jacob Blaustein Institutes for Desert Research, Ben Gurion University of the Negev, Sede-Boker Campus 8499000, Israel.
J Fungi (Basel). 2021 Feb 1;7(2):100. doi: 10.3390/jof7020100.
The blastocladialean fungus parasitizes three microalgae species of economic interest: , and . For the first time, we characterized the developmental stages of isolated fungal propagules in co-culture, finding a generation time of 16 h. We established a patho-system to compare the infection in the three different host species for 48 h, with two different setups to quantify parameters of the infection and parameters of the parasite fitness. The prevalence of the parasite in and cultures was 100%, but only 20% in culture. The infection of not only reached lower prevalence but was also qualitatively different; the infection developed preferentially on senescent cells and more resting cysts were produced, being consistent with a reservoir host. In addition, we carried out cross infection experiments and the inoculation of a mixed algal culture containing the three microalgae, to determine the susceptibility of the host species and to investigate the preference of for these microalgae. The three tested microalgae showed different susceptibility to , which correlates with blastoclad's preference to the host in the following order: > > .
[具体微藻物种1]、[具体微藻物种2]和[具体微藻物种3]。我们首次对共培养中分离出的真菌繁殖体的发育阶段进行了表征，发现其世代时间为16小时。我们建立了一个病理系统，以比较在48小时内三种不同宿主物种中的感染情况，并采用两种不同的设置来量化感染参数和寄生虫适应性参数。寄生虫在[具体微藻物种1]和[具体微藻物种2]培养物中的患病率为100%，但在[具体微藻物种3]培养物中仅为20%。[具体微藻物种3]的感染不仅患病率较低，而且在性质上也有所不同；感染优先在衰老细胞上发展，并且产生了更多的静息囊肿，这与储存宿主一致。此外，我们进行了交叉感染实验以及接种含有这三种微藻的混合藻类培养物，以确定宿主物种的易感性，并研究[芽枝霉]对这些微藻的偏好。三种受试微藻对[芽枝霉]表现出不同的易感性，这与芽枝霉对宿主的偏好顺序相关：[具体微藻物种1]>[具体微藻物种2]>[具体微藻物种3]。
