Moretti Riccardo, Soglia Dominga, Chessa Stefania, Sartore Stefano, Finocchiaro Raffaella, Rasero Roberto, Sacchi Paola
Department of Veterinary Science, University of Turin, 10095 Grugliasco (Turin), Italy.
Associazione Nazionale Allevatori Razza Frisona e Jersey Italiana-ANAFIJ, 26100 Cremona, Italy.
Animals (Basel). 2021 Feb 1;11(2):366. doi: 10.3390/ani11020366.
Mastitis is an infectious disease affecting the mammary gland, leading to inflammatory reactions and to heavy economic losses due to milk production decrease. One possible way to tackle the antimicrobial resistance issue stemming from antimicrobial therapy is to select animals with a genetic resistance to this disease. Therefore, aim of this study was to analyze the genetic variability of the SNPs found in candidate genes related to mastitis resistance in Holstein Friesian bulls. Target regions were amplified, sequenced by Next-Generation Sequencing technology on the Illumina MiSeq, and then analyzed to find correlation with mastitis related phenotypes in 95 Italian Holstein bulls chosen with the aid of a selective genotyping approach. On a total of 557 detected mutations, 61 showed different genotype distribution in the tails of the deregressed EBVs for SCS and 15 were identified as significantly associated with the phenotype using two different approaches. The significant SNPs were identified in intergenic or intronic regions of six genes, known to be key components in the immune system (namely , , , , and ). These SNPs could be considered as candidates for a future genetic selection for mastitis resistance, although further studies are required to assess their presence in other dairy cattle breeds and their possible negative correlation with other traits.
乳腺炎是一种影响乳腺的传染病，会引发炎症反应，并因产奶量下降导致巨大的经济损失。解决抗菌治疗引起的抗菌药物耐药性问题的一种可能方法是选择对该疾病具有遗传抗性的动物。因此，本研究的目的是分析荷斯坦弗里生公牛中与乳腺炎抗性相关的候选基因中发现的单核苷酸多态性（SNP）的遗传变异性。通过下一代测序技术在Illumina MiSeq上对目标区域进行扩增、测序，然后借助选择性基因分型方法对95头意大利荷斯坦公牛进行分析，以找出与乳腺炎相关表型的相关性。在总共检测到的557个突变中，有61个在体细胞评分（SCS）的去回归估计育种值（EBV）的尾部显示出不同的基因型分布，并且使用两种不同方法确定有15个与表型显著相关。在六个基因的基因间或内含子区域中鉴定出了显著的SNP，这些基因是免疫系统的关键组成部分（即 、 、 、 、 和 ）。这些SNP可被视为未来乳腺炎抗性基因选择的候选基因，尽管需要进一步研究来评估它们在其他奶牛品种中的存在情况以及它们与其他性状可能存在的负相关性。
