Laboratory of Cancer Chemoprevention and Oncopharmacology, N.N. Petrov National Medical Research Center of Oncology, St. Petersburg, Russian Federation.
Laboratory of Carcinogenesis and Aging, N.N. Petrov National Medical Research Center of Oncology, St. Petersburg, Russian Federation.
Curr Aging Sci. 2021;14(2):124-132. doi: 10.2174/1874609814666210203091803.
The aim of the experiments was to find out the factors on which age-related sensitivity to the occurrence of BPH depends.
45 Male Wistar rats aged 3 and 24 months were used. In each age group, there were intact rats and animals with induced BPH (by surgical castration + testosterone injections, 25 mg/kg x 7). On the 36th day of the experiment, blood was taken from rats to determine serum testosterone, cholesterol, triglycerides and glucose; then, the animals were autopsied, their prostates were weighed, and their morphology was studied.
Young mature intact rats had much higher testosterone levels (6.2±0.93 nmol/l) than old intact (3.8±0.55 nmol/l), while the ratio of prostate weight was inverse. The weight of the prostate and prostatic index in old rats with induced BPH was significantly higher not only in comparison with the old intact rats but also with young animals after BPH induction. Morphologically, the inflammatory foci were determined not only in the prostates of old rats, which induced BPH, but also in intact animals. Besides, in old intact rats, the foci of prostate hyperplasia were often noted.
Our experimental model indicates the important role of non-bacterial prostatitis in the pathogenesis of BPH. No metabolic disorders in BPH induction were revealed. The sensitivity of the prostate of old rats to BPH development is increasing despite the low concentrations of testosterone in the body. Age sensitivity to BPH is probably determined by a higher expression of androgen receptors in old animals.
实验目的是找出与年龄相关的 BPH 易感性相关的因素。
使用 3 月龄和 24 月龄雄性 Wistar 大鼠 45 只。在每个年龄组中，均有完整大鼠和诱导 BPH 动物（通过手术去势+睾酮注射，25mg/kg x 7）。在实验的第 36 天，从大鼠中取血以测定血清睾酮、胆固醇、甘油三酯和葡萄糖；然后，对动物进行解剖，称重前列腺，并研究其形态。
年轻成熟的完整大鼠的睾酮水平（6.2±0.93nmol/l）明显高于老年完整大鼠（3.8±0.55nmol/l），而前列腺重量比则相反。诱导 BPH 的老年大鼠的前列腺重量和前列腺指数不仅明显高于老年完整大鼠，而且高于诱导 BPH 后的年轻动物。形态学上，不仅在诱导 BPH 的老年大鼠的前列腺中，而且在完整动物的前列腺中都确定了炎症灶。此外，在老年完整大鼠中，还经常观察到前列腺增生的病灶。
我们的实验模型表明非细菌性前列腺炎在 BPH 发病机制中起重要作用。在诱导 BPH 中未发现代谢紊乱。尽管体内睾酮浓度较低，但老年大鼠前列腺对 BPH 发展的敏感性增加。老年对 BPH 的敏感性可能是由老年动物中雄激素受体的高表达决定的。
